Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training

Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training

Author Remedio, Rafael Neodini Google Scholar
Barbosa, Rodrigo Avelaira Google Scholar
Castellar, Alexandre Google Scholar
Gomes, Ricardo José Autor UNIFESP Google Scholar
Caetano, Flavio Henrique Google Scholar
Institution São Paulo State Univ UNESP
Universidade Federal de São Paulo (UNIFESP)
Abstract Alterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. the practice of physical training and its morphological effects in this organ, however, are scarcely studied. in order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. in conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Diabetes
Physical training
Liver
Histochemistry
Ultrastructure
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-08-01
Published in Tissue & Cell. Edinburgh: Churchill Livingstone, v. 43, n. 4, p. 207-215, 2011.
ISSN 0040-8166 (Sherpa/Romeo, impact factor)
Publisher Churchill Livingstone
Extent 207-215
Origin https://dx.doi.org/10.1016/j.tice.2011.03.003
Access rights Closed access
Type Article
Web of Science ID WOS:000208738500001
URI https://repositorio.unifesp.br/handle/11600/33932

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