Dual Effect of Low-Level Laser Therapy (LLLT) on the Acute Lung Inflammation Induced by Intestinal Ischemia and Reperfusion: Action on Anti- and Pro-Inflammatory Cytokines

Dual Effect of Low-Level Laser Therapy (LLLT) on the Acute Lung Inflammation Induced by Intestinal Ischemia and Reperfusion: Action on Anti- and Pro-Inflammatory Cytokines

Author Mafra de Lima, F. Google Scholar
Villaverde, A. B. Google Scholar
Albertini, R. Google Scholar
Correa, J. C. Google Scholar
Carvalho, R. L. P. Google Scholar
Munin, E. Google Scholar
Araujo, T. Google Scholar
Silva, J. A. Google Scholar
Aimbire, F. Autor UNIFESP Google Scholar
Institution Univap
Unicastelo
Nove de Julho Univ
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background and Objective: It is unknown if pro-and anti-inflammatory mediators in acute lung inflammation induced by intestinal ischemia and reperfusion (i-I/R) can be modulated by low-level laser therapy (LLLT).Study Design/Material and Methods: A controlled ex vivo study was developed in which rats were irradiated (660 nm, 30 mW, 0.08 cm(2) of spot size) on the skin over the right upper bronchus 1 hour post-mesenteric artery occlusion and euthanized 4 hours later. for pretreatment with anti-tumor necrosis factor (TNF) or IL-10 antibodies, the rats received either one of the agents 15 minutes before the beginning of reperfusion.Methods: Lung edema was measured by the Evans blue extravasation and pulmonary neutrophils influx was determined by myeloperoxidase (MPO) activity. Both TNF and IL-10 expression and protein in lung were evaluated by RT-PCR and ELISA, respectively.Results: LLLT reduced the edema (80.1 +/- 41.8 mu g g(-1) dry weight), neutrophils influx (0.83 +/- 0.02 x 10(6) cells ml(-1)), MPO activity (2.91 +/- 0.60), and TNF (153.0 +/- 21.0 pg mg(-1) tissue) in lung when compared with respective control groups. Surprisingly, the LLLT increased the IL-10 (0.65 +/- 0.13) in lung from animals subjected to i-I/R. Moreover, LLLT (0.32 +/- 0.07 pg ml(-1)) reduced the TNF-alpha level in RPAECs when compared with i-I/R group. the presence of anti-TNF or IL-10 antibodies did not alter the LLLT effect on IL-10 (465.1 +/- 21.0 pg mg (1) tissue) or TNF (223.5 +/- 21.0 pg mg (1) tissue) in lung from animals submitted to i-I/R.Conclusion: the results indicate that the LLLT attenuates the i-I/R-induced acute lung inflammation which favor the IL-10 production and reduce TNF generation. Lasers Surg. Med. 43:410-420, 2011. (C) 2011 Wiley-Liss, Inc.
Keywords phototherapy
cytokines
mesenteric ischemia
reperfusion injury
IL-10
TNF
inflammation
Language English
Date 2011-07-01
Published in Lasers in Surgery and Medicine. Malden: Wiley-Blackwell, v. 43, n. 5, p. 410-420, 2011.
ISSN 0196-8092 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 410-420
Origin http://dx.doi.org/10.1002/lsm.21053
Access rights Closed access
Type Article
Web of Science ID WOS:000292470000007
URI http://repositorio.unifesp.br/handle/11600/33842

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