Low-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndrome

Low-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndrome

Author Lima, Flavia Mafra de Google Scholar
Moreira, Leonardo M. Google Scholar
Villaverde, A. B. Google Scholar
Albertini, Regiane Google Scholar
Castro-Faria-Neto, Hugo C. Google Scholar
Aimbire, Flavio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Res & Dev Inst IP&D
Dept Biosyst Engn
Unicastelo
Ctr Univ Nove Julho Uninove
Fiocruz MS
Abstract The aim of this work was to investigate if the low-level laser therapy (LLLT) on acute lung inflammation (ALI) induced by lipopolysaccharide (LPS) is linked to tumor necrosis factor (TNF) in alveolar macrophages (AM) from bronchoalveolar lavage fluid (BALF) of mice. LLLT has been reported to actuate positively for relieving the late and early symptoms of airway and lung inflammation. It is not known if the increased TNF mRNA expression and dysfunction of cAMP generation observed in ALI can be influenced by LLLT. for in vivo studies, Balb/c mice (n = 5 for group) received LPS inhalation or TNF intra nasal instillation and 3 h after LPS or TNF-alpha, leukocytes in BALF were analyzed. LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 660 nm and a dose of 4.5 J/cm(2). the mice were irradiated 15 min after ALI induction. in vitro AM from mice were cultured for analyses of TNF mRNA expression and protein and adenosine3':5'-cyclic monophosphate (cAMP) levels. One hour after LPS, the TNF and cAMP levels in AM were measured by ELISA. RT-PCR was used to measure TNF mRNA in AM. the LLLT was inefficient in potentiating the rolipram effect in presence of a TNF synthesis inhibitor. LLLT attenuated the neutrophil influx and TNF in BALF. in AM, the laser increased the cAMP and reduced the TNF-alpha mRNA. LLLT increases indirectly the cAMP in AM by a TNF-dependent mechanism.
Keywords Acute lung inflammation
Alveolar macrophages
Cyclic AMP
TNF
Low-level laser therapy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2008/08048-4
FAPESP: 2007/02596-7
Date 2011-05-01
Published in Lasers in Medical Science. London: Springer London Ltd, v. 26, n. 3, p. 389-400, 2011.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 389-400
Origin http://dx.doi.org/10.1007/s10103-010-0874-x
Access rights Closed access
Type Article
Web of Science ID WOS:000289109800016
URI http://repositorio.unifesp.br/handle/11600/33665

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