Poliovirus 3C proteinase inhibition by organotelluranes

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Gouvea, Iuri E. [UNIFESP]
Santos, Jorge A. N. [UNIFESP]
Burlandy, Fernanda M.
Tersariol, Ivarne L. S.
Silva, Edson E. da
Juliano, Maria A. [UNIFESP]
Juliano, Luiz [UNIFESP]
Cunha, Rodrigo L. O. R. [UNIFESP]
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The 3C proteinase, essential for human poliovirus (PV) replication, has unique characteristics as its three-dimensional structure resembles chymotrypsin, but its catalytic nucleophile is a cysteine SH group rather than the OH group of serine. Here, we describe the use of tellurium compounds as inhibitors of PV3C proteinase. A rapid, stoichiometric and covalent inactivation of PV3C was observed with both a chloro-telluroxetane and a bis-vinylic organotellurane. These compounds also inhibit human cathepsins B, L, S, and K with second order rate constants higher than those obtained for PV3C. Chloro-telluroxetane inhibits replication of PV in human embryonic rhabdomyosarcoma cells in the low micromolar range and below the toxic level for the host cells. Bis-vinylic organotellurane is more effective as antiviral agent but reduces the cell viability by 20% at 10 mM, a concentration almost completely inhibiting virus growth. This is the first description of inhibition of viral 3C proteinase with antiviral property by this class of compounds.
Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 392, n. 6, p. 587-591, 2011.