Protein tyrosine phosphatase alpha regulates cell detachment and cell death profiles induced by nitric oxide donors in the A(431) human carcinoma cell line

Protein tyrosine phosphatase alpha regulates cell detachment and cell death profiles induced by nitric oxide donors in the A(431) human carcinoma cell line

Author Costa, Paulo E. da Autor UNIFESP Google Scholar
Batista, Wagner L. Autor UNIFESP Google Scholar
Curcio, Marli F. Autor UNIFESP Google Scholar
Moraes, Miriam S. Autor UNIFESP Google Scholar
Borges, Roberta Eller Autor UNIFESP Google Scholar
Nascimento, Patricia A. Autor UNIFESP Google Scholar
Travassos, Luiz R. Autor UNIFESP Google Scholar
Monteiro, Hugo P. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We investigated the role of protein tyrosine phosphatase-alpha (PTP alpha) expression in the cell death profile of the A431 human carcinoma cell line that was induced by cytotoxic concentrations of the nitric oxide (NO) donors sodium nitroprusside (SNP) and 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18). Both NO donors promoted extensive cell detachment in A431 parental cells as compared to the detachment observed for A431 cells that ectopically expressed PTP alpha (A431 (A27B(PTP alpha)) cells). the NO-induced cell death characteristics for both cell lines were examined. After incubation for 10 hours with 2.0 mM SNP, attached or detached A431 cells underwent apoptosis. Cells were highly positive for Annexin-V, featured increased cleavage of procaspase-8, activation of downstream caspase-3, and activation of poly-ADP-ribose polymerase 1 (PARP-1). in contrast, exposure of A431 (A27B(PTP alpha)) cells to 2.0 mM SNP produced an increase in the release of lactate dehydrogenase and enhanced incorporation of propidium iodide. in addition, A431 (A27B(PTP alpha)) cells showed partial inhibition of the activities of caspase-8, caspase-3, and PARP-1 upon detachment and cell death induced by SNP treatment. Results indicate that necrotic cell damage was induced, characterized by cellular swelling and lysis. We conclude from these results that PTP alpha regulates the A431 tumor cell death profile mediated by NO donors. Expression of PTP alpha or its absence may determine the occurrence of NO-induced cell death with necrotic or apoptotic features, respectively.
Keywords A431 tumor cells
Cell death
Epidermal growth factor
Nitric oxide donors
Protein tyrosine phosphatase-alpha
Sodium nitroprusside
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 06/56311-0
FAPESP: 07/59617-6
CNPq: 42011/2005
Date 2011-01-01
Published in Redox Report. Leeds: Maney Publishing, v. 16, n. 1, p. 27-37, 2011.
ISSN 1351-0002 (Sherpa/Romeo, impact factor)
Publisher Maney Publishing
Extent 27-37
Origin http://dx.doi.org/10.1179/174329211X12968219310792
Access rights Closed access
Type Article
Web of Science ID WOS:000290614000005
URI http://repositorio.unifesp.br/handle/11600/33341

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