Survivin and Telomerase Expression in the Uterine Cervix of Women With Human Papillomavirus-Induced Lesions

Survivin and Telomerase Expression in the Uterine Cervix of Women With Human Papillomavirus-Induced Lesions

Author Resende Barbosa, Lyliana Coutinho Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Correa, Jose Carlos Google Scholar
Ribalta, Julisa Chamorro Lascasas Autor UNIFESP Google Scholar
Institution Univ Vale Sapucai UNIVAS
Universidade Federal de São Paulo (UNIFESP)
Inst Pathol Jose Carlos Correa
Abstract Introduction: Infection by human papillomavirus is the most important risk factor in the pathogenesis of uterine cervical cancer. the aims of this study were to evaluate the expression of survivin protein and telomerase enzyme in samples of uterine cervix from women with human papillomavirus-induced lesions and to determine the relationship between survivin and telomerase expression and the different grades of cervical squamous intraepithelial neoplasia and invasive cervical carcinoma.Methods: Biopsy samples from the uterine cervix of 105 women aged 18 to 80 years were analyzed. the patients were divided into 5 groups: WN group, 20 patients without neoplasia; CIN-1 group, 24 patients with grade 1 cervical intraepithelial neoplasia (CIN), grade 1; CIN-2 group, 20 patients with CIN grade 2; CIN-3 group, 24 patients with CIN, grade 3; and ICC group, 17 patients with invasive cervical carcinoma. Human papillomavirus detection, telomerase activity, and survivin expression were assessed using polymerase chain reaction (PCR), real-time PCR (RT-PCR), and immunochemistry, respectively.Results: There was a significant increase in the expression of telomerase and survivin associated with the severity of the lesion.Conclusions: the results suggest that mechanisms that promote both cell proliferation (telomerase activity) and cell survival (survivin expression) are active in cervical cancer and its precursor lesions. There was a negative correlation between survivin expression and the number of PCR cycles necessary to detect telomerase activity in the total sample, achieving statistical significance in patients in the CIN-3 group.
Keywords Telomerase
Survivin protein
Human papillomavirus
Uterine cervical cancer
Cervical intraepithelial neoplasia
Language English
Date 2011-01-01
Published in International Journal of Gynecological Cancer. Philadelphia: Lippincott Williams & Wilkins, v. 21, n. 1, p. 15-21, 2011.
ISSN 1048-891X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 15-21
Origin http://dx.doi.org/10.1097/IGC.0b013e318203d42b
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000285582500004
URI http://repositorio.unifesp.br/handle/11600/33195

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