ACE2-angiotensin-(1-7)-Mas axis in renal ischaemia/reperfusion injury in rats
Silveira, Katia D. da
Bosco, Kenia S. Pompermayer
Diniz, Lucio R. L.
Carmona, Adriana Karaoglanovic [UNIFESP]
Cassali, Giovanni D.
Sousa, Lirlandia P. de
Teixeira, Mauro M.
Santos, Robson A. S.
Simoes e Silva, Ana C.
Ribeiro Vieira, Maria A.
Is part ofClinical Science
MetadataShow full item record
AngII (angiotensin II), ACE (angiotensin I-converting enzyme) and the AT(1) receptor (AngII type I receptor) are associated with the inflammatory process and microvascular dysfunction of AKI (acute kidney injury) induced by renal I/R (ischaemia/reperfusion). However, Ang-(1-7) [angiotensin-(1-7)], ACE2 (angiotensin I-converting enzyme 2) and the Mas receptor also play a role in renal disease models. Therefore, in the present study, we have examined the renal profile of Ang-(1-7), ACE2 and the Mas receptor in renal I/R and compared them with that of AngII, ACE and the AT(1) receptor. Male Wistar rats were submitted to left nephrectomy and ischaemia (45 min) followed by reperfusion (2 or 4 h) in the right kidney. At 4 h of reperfusion, renal AngII was increased (P < 0.01) and renal Ang-(1-7) was decreased substantially (P < 0.05), although plasma levels of both angiotensins were unchanged. in addition, renal I/R decreased the renal mRNA expression of renin (P < 0.05), AT(1) receptors (P < 0.001) and ACE2 (P < 0.05). At 2 and 4 h of reperfusion, renal ACE activity was reduced (P < 0.05). On the other hand, renal expression of the Mas receptor was greatly increased at 4 h of reperfusion (P < 0.01), which was confirmed by immunohistochemical and Western blot analysis. in conclusion, increased renal expression of the Mas receptor associated with changes in the RAS (renin-angiotensin-system)-related peptidases support an important role for the ACE2 Ang-(1-7) Mas axis in AKI.
CitationClinical Science. London: Portland Press Ltd, v. 119, n. 9-10, p. 385-394, 2010.
Keywordsacute kidney injury
angiotensin I-converting enzyme (ACE)
angiotensin I-converting enzyme 2 (ACE2)
angiotensin II (AngII)
SponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
- EPM - Artigos 
Showing items related by title, author, creator and subject.
Aerobic Exercise Training-Induced Left Ventricular Hypertrophy Involves Regulatory MicroRNAs, Decreased Angiotensin-Converting Enzyme-Angiotensin II, and Synergistic Regulation of Angiotensin-Converting Enzyme 2-Angiotensin (1-7) Fernandes, Tiago; Hashimoto, Nara Y.; Magalhaes, Flavio C.; Fernandes, Fernanda Barrinha [UNIFESP]; Casarini, Dulce Elena [UNIFESP]; Carmona, Adriana Karaoglanovic [UNIFESP]; Krieger, Jose E.; Phillips, M. Ian; Oliveira, Edilamar M. (Lippincott Williams & Wilkins, 2011-08-01)Aerobic exercise training leads to a physiological, nonpathological left ventricular hypertrophy; however, the underlying biochemical and molecular mechanisms of physiological left ventricular hypertrophy are unknown. the ...
New specific angiotensin antagonists - [8-valine]-angiotensins i, [8-isoleucine]-angiotensins I, and chlorambucil-des-1-aspartic,8-valine-angiotensins I Paiva, Antonio Cechelli de Mattos [UNIFESP]; NOUAILHE.VL; Miyamoto, M. E.; Bergsten-Mendes, Gun [UNIFESP]; Paiva, Therezinha Bandiera [UNIFESP] (Amer Chemical Soc, 1973-01-01)
Long-Lasting Inhibition of Angiotensin Response in Rats by Depot Administration of Octanoyl-[Leu8]-Angiotensin II Abdulkader, Regina C.; Silva, Helio B.; Marcondes, Marcello; Vasconcelos, Eleonidas A.; Paiva, Antonio CM (Royal Pharmaceutical Soc Great Britain, 1979-01-01)