Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide
Zeferino, Eduardo Gregatto
Bueno, Carlos Eduardo da Silveira
Oyama, Lila Missae [UNIFESP]
Ribeiro, Daniel Araki [UNIFESP]
Is part ofInternational Endodontic Journal
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P>AimsTo evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo.MethodologyAliquots of 1 x 104 murine fibroblasts were incubated at 37 degrees C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 mu g mL-1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test.ResultsMineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement.ConclusionWhite MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.
CitationInternational Endodontic Journal. Malden: Wiley-Blackwell, v. 43, n. 10, p. 843-848, 2010.
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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