Effect of doxorubicin on cytokine production by lymphocytes and the Th1/Th2 balance

Effect of doxorubicin on cytokine production by lymphocytes and the Th1/Th2 balance

Author Santos, Ronaldo Vagner Thomatieli dos Autor UNIFESP Google Scholar
Caperuto, E. C. Google Scholar
Mello, Marco Tulio de Autor UNIFESP Google Scholar
Costa Rosa, L. F. B. P. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Doxorubicin (DOXO) is a potent chemotherapeutic used mainly against solid tumours; however, it has several side effects that can limit its clinical use. On the other hand, the effect of DOXO upon lymphocyte function is controversial. Some studies demonstrate that DOXO administration in vitro suppresses T-cell activation, while the cellular function has been shown to increase in vitro. the objective of this study was to investigate the effect of DOXO on lymphocyte cytokine production in rats. the animals were divided into: SAL (control, n = 10) and DOX (DOXO treated, n = 10). the DOX group received only one DOXO dose at 15 kg Kg(-1) by intraperitoneal injection. Forty-eight hours after DOXO administration, the animals were killed by decapitation. IL-2 production was significantly enhanced (p < 0.05) in lymphocytes from rats treated with DOXO (169.17 +/- 21.73 pg mL 10(5) cell) as compared to cells from SAL (45.92 +/- 10.53 pg mL 10(5) cell). the administration of DOXO decreased (<0.05) IL-4 production in the DOXO group (29.85 +/- 13.09 pg mL 10(5) cell) relative to the SAL group (75.08 +/- 15.31 pg mL 10(5) cell). the IL-2/IL-4 ratio was higher (<0.05) in the DOX group (5.99 +/- 0.44), as compared to SAL group (0.73 +/- 0.12). in conclusion, our results suggest that a dose of DOXO promotes an alteration in the Th1/Th2 balance, promoting a shift towards a Th1-dominant cytokine response. (C) 2010 Elsevier Masson SAS. All rights reserved.
Keywords Doxorubicin
Adryamicin
IL-2
IL-4
Lymphocytes
Th1/Th2 balance
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 01/13766-4
Date 2010-10-01
Published in Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 64, n. 8, p. 579-581, 2010.
ISSN 0753-3322 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 579-581
Origin https://dx.doi.org/10.1016/j.biopha.2010.02.002
Access rights Closed access
Type Article
Web of Science ID WOS:000283571100013
URI https://repositorio.unifesp.br/handle/11600/32952

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