Effect of plant neutrophil elastase inhibitor on leucocyte migration, adhesion and cytokine release in inflammatory conditions

Effect of plant neutrophil elastase inhibitor on leucocyte migration, adhesion and cytokine release in inflammatory conditions

Author Oliveira, C. Autor UNIFESP Google Scholar
Navarro-Xavier, R. A. Google Scholar
Anjos-Vallota, E. A. Google Scholar
Martins, J. O. Google Scholar
Silveira, Vera Lucia Flor Autor UNIFESP Google Scholar
Goncalves, L. R. C. Google Scholar
Araujo, M. S. Autor UNIFESP Google Scholar
Motta, G. Autor UNIFESP Google Scholar
Sannomiya, P. Google Scholar
Oliva, Maria Luiza Vilela Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Oswaldo Cruz
Universidade de São Paulo (USP)
Inst Butantan
Abstract BACKGROUND and PURPOSEThe serine and cysteine peptidase inhibitor, BbCI, isolated from Bauhinia bauhinioides seeds, is similar to the classical plant Kunitz inhibitor, STI, but lacks disulphide bridges and methionine residues. BbCI blocks activity of the serine peptidases, elastase (K(iapp) 5.3 nM) and cathepsin G (K(iapp) 160.0 nM), and the cysteine peptidase cathepsin L (K(iapp) 0.2 nM). These three peptidases play important roles in the inflammatory process.EXPERIMENTAL APPROACHWe measured the effects of BbCI on paw oedema and on leucocyte accumulation in pleurisy, both induced by carrageenan. Leucocyte-endothelial cell interactions in scrotal microvasculature in Wistar rats were investigated using intravital microscopy. Cytokine levels in pleural exudate and serum were measured by ELISA.KEY RESULTSPretreatment of the animals with BbCI (2.5 mg.kg(-1)), 30 min before carrageenan-induced inflammation, effectively reduced paw oedema and bradykinin release, neutrophil migration into the pleural cavity. the number of rolling, adhered and migrated leucocytes at the spermatic fascia microcirculation following carrageenan injection into the scrotum were reduced by BbCI pretreatment. Furthermore, levels of the rat chemokine cytokine-induced neutrophil chemo-attractant-1 were significantly reduced in both pleural exudates and serum from animals pretreated with BbCI. Levels of interleukin-1 beta or tumour necrosis factor-alpha, however, did not change.CONCLUSIONS and IMPLICATIONSTaken together, our data suggest that the anti-inflammatory properties of BbCI may be useful in investigations of other pathological processes in which human neutrophil elastase, cathepsin G and cathepsin L play important roles.
Keywords Bauhinia
intravital microscopy
Kunitz inhibitor
paw oedema
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-10-01
Published in British Journal of Pharmacology. Malden: Wiley-Blackwell, v. 161, n. 4, p. 899-910, 2010.
ISSN 0007-1188 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 899-910
Origin http://dx.doi.org/10.1111/j.1476-5381.2010.00924.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000282179000014
URI http://repositorio.unifesp.br/handle/11600/32924

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