Proinflammatory and Anti-inflammatory Cytokines Present in the Acute Phase of Experimental Colitis Treated with Saccharomyces boulardii
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2010-09-01
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Grijo, Nathalia Nahas [UNIFESP]
Borra, Ricardo Carneiro
Sdepanian, Vera Lucia [UNIFESP]
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To study the proinflammatory and anti-inflammatory cytokines present in the acute phase of trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis treated with Saccharomyces boulardii.Thirty male Wistar rats were divided into three groups: (1) treated group-received Saccharomyces boulardii for 14 days; (2) non-treated group-received sodium chloride solution for 14 days; (3) control group. Colitis was induced on the seventh day of the study in the treated and the non-treated groups using TNBS (10 mg) dissolved in 50% ethanol. Quantification of cytokines, including interleukin (IL)-1 beta (IL-1 beta), IL-6, transforming growth factor-beta (TGF-beta), IL-10 and tumor necrosis factor-alpha (TNF-alpha), in the serum and colonic tissue collected on day 14 were carried out using an enzyme-linked immunosorbent assay (ELISA).The mean concentrations of TGF-beta in both the serum and the colonic tissue of the treated group were statistically higher than that of the control group. the mean concentration of TGF-beta in the colonic tissue of the non-treated group was also statistically higher than the control group.The group treated with Saccharomyces boulardii showed increased amounts of TGF-beta, an anti-inflammatory cytokine, during the acute phase of colitis. There were no differences in the amount of TNF-alpha, IL-1 beta, IL-6, and IL-10 between the treated and the non-treated or the control groups during the acute phase of experimental colitis induced by TNBS.
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Digestive Diseases and Sciences. Dordrecht: Springer, v. 55, n. 9, p. 2498-2504, 2010.