Show simple item record

dc.contributor.authorSantos, G. A. dos
dc.contributor.authorThomé, Carolina Hassibe [UNIFESP]
dc.contributor.authorFerreira, Germano A
dc.contributor.authorYoneda, Juliana Sakamoto
dc.contributor.authorNobre, Thatyane Morimoto
dc.contributor.authorPerez, Katia Regina [UNIFESP]
dc.contributor.authorScheucher, Priscila Santos
dc.contributor.authorGimenes-Teixeira, Hamilton Luiz
dc.contributor.authorConstantino, Maurício Gomes
dc.contributor.authorOliveira, Kleber Thiago de
dc.contributor.authorFaça, Vítor Marcel
dc.contributor.authorFalcão, Roberto Passetto
dc.contributor.authorGreene, Lewis Joel [UNIFESP]
dc.contributor.authorRego, Eduardo Magalhães
dc.contributor.authorCiancaglini, Pietro
dc.date.accessioned2016-01-24T14:05:21Z
dc.date.available2016-01-24T14:05:21Z
dc.date.issued2010-09-01
dc.identifierhttp://dx.doi.org/10.1016/j.bbamem.2010.05.013
dc.identifier.citationBiochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1798, n. 9, p. 1714-1723, 2010.
dc.identifier.issn0005-2736
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32834
dc.description.abstract10-(Octyloxy) decyl-2-(trimethylammonium) ethyl phosphate (ODPC) is an alkylphospholipid that can interact with cell membranes because of its amphiphilic character. We describe here the interaction of ODPC with liposomes and its toxicity to leukemic cells with an ED-50 of 5.4, 5.6 and 2.9 pM for 72 h of treatment for inhibition of proliferation of NB4, U937 and K562 cell lines, respectively, and lack of toxicity to normal hematopoietic progenitor cells at concentrations up to 25 pM. the ED-50 for the non-malignant HEK-293 and primary human umbilical vein endothelial cells (HUVEC) was 63.4 and 60.7 mu M, respectively. the critical micellar concentration (CMC) of ODPC was 200 mu M. Dynamic light scattering indicated that dipalmitoylphosphatidylcholine (DPPC) liposome size was affected only above the CMC of ODPC. Differential calorimetric scanning (DCS) of liposomes indicated a critical transition temperature (T(c)) of 41.5 degrees C and an enthalpy (Delta H) variation of 7.3 kcal mol(-1). the presence of 25 mu M ODPC decreased T(c) and Delta H to 393 degrees C and 4.7 kcal mol(-1), respectively. ODPC at 250 mu M destabilized the liposomes (36.3 degrees C. 0.46 kcal mol(-1)). Kinetics of 5(6)-carboxyfluorescein (CF) leakage from different liposome systems indicated that the rate and extent of CF release depended on liposome composition and ODPC concentration and that above the CMC it was instantaneous. Overall, the data indicate that ODPC acts on in vitro membrane systems and leukemia cell lines at concentrations below its CMC, suggesting that it does not act as a detergent and that this effect is dependent on membrane composition. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundacao de Apoio ao Ensino
dc.description.sponsorshipPesquisa e Assistencia do Hospital das Clinicas da FMRP-USP (FAEPA)
dc.format.extent1714-1723
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica Et Biophysica Acta-biomembranes
dc.rightsAcesso aberto
dc.subject10-(Octyloxy) decyl-2-(trimethylammonium)en
dc.subjectethyl phosphateen
dc.subjectLipidsen
dc.subjectLiposomeen
dc.subjectAlkylphospholipidsen
dc.subjectLeukemiaen
dc.titleInteraction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cellsen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Nacl Ciencia & Tecnol Celulas Tronco & Terap
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.description.affiliationUSP, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationInst Nacl Ciencia & Tecnol Celulas Tronco & Terap, São Paulo, Brazil
dc.description.affiliationUSP, Fac Med Ribeirao Preto, Dept Clin Med, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04039002 São Paulo, Brazil
dc.description.affiliationUSP, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationUSP, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04039002 São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2008/06619-4
dc.identifier.fileWOS000280752100009.pdf
dc.identifier.doi10.1016/j.bbamem.2010.05.013
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000280752100009


Files in this item

This item appears in the following Collection(s)

Show simple item record