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dc.contributor.authorSilva, Erick J. R. [UNIFESP]
dc.contributor.authorQueiroz, Daniel B. C. [UNIFESP]
dc.contributor.authorHonda, Luciana [UNIFESP]
dc.contributor.authorAvellar, Maria Christina W. [UNIFESP]
dc.identifier.citationMolecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 325, n. 1-2, p. 64-77, 2010.
dc.description.abstractGlucocorticoids regulate several physiological functions, including reproduction, in mammals. Curiously, little is known about glucocorticoid-induced effects on the epididymis, an androgen-dependent tissue with vital role on sperm maturation. Here, RT-PCR, Western blot and immunohistochemical studies were performed to evaluate expression, cellular distribution and hormonal regulation of glucocorticoid receptor (GR) along rat epididymis. the rat orthologue of human GR alpha (mRNA and protein) was detected in caput, corpus and cauda epididymis and immunolocalized in the nucleus and cytoplasm of different epididymal cells (epithelial, smooth muscle and interstitial cells) and nerve fibers. Changes in plasma glucocorticoid and androgen levels differentially regulated GR expression in caput and cauda epididymis by homologous and heterologous mechanisms. in vivo treatment with dexamethasone significantly changed the expression of glucocorticoid-responsive genes and induced ligand-dependent GR nuclear translocation in epithelial cells from epididymis, indicating that GR is fully active in this tissue. Heterologous regulation of androgen receptor expression by glucocorticoids was also demonstrated in cauda epididymis. Our results demonstrate that the epididymis is under glucocorticoid regulation, opening new insights into the roles of this hormone in male fertility. (C) 2010 Elsevier Ireland Ltd. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFogarty International Center, USA
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsAcesso restrito
dc.subjectGlucocorticoid receptoren
dc.subjectGene regulationen
dc.titleGlucocorticoid receptor in the rat epididymis: Expression, cellular distribution and regulation by steroid hormonesen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 05/55738-8
dc.description.sponsorshipIDCNPq: 475238/2006-0
dc.description.sponsorshipIDFogarty International Center, USA: D43TW/HD00627
dc.description.sponsorshipIDFogarty International Center, USA: Unifesp/UNC 5-53284
dc.description.sourceWeb of Science

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