Glucocorticoid receptor in the rat epididymis: Expression, cellular distribution and regulation by steroid hormones

Glucocorticoid receptor in the rat epididymis: Expression, cellular distribution and regulation by steroid hormones

Author Silva, Erick J. R. Autor UNIFESP Google Scholar
Queiroz, Daniel B. C. Autor UNIFESP Google Scholar
Honda, Luciana Autor UNIFESP Google Scholar
Avellar, Maria Christina W. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Glucocorticoids regulate several physiological functions, including reproduction, in mammals. Curiously, little is known about glucocorticoid-induced effects on the epididymis, an androgen-dependent tissue with vital role on sperm maturation. Here, RT-PCR, Western blot and immunohistochemical studies were performed to evaluate expression, cellular distribution and hormonal regulation of glucocorticoid receptor (GR) along rat epididymis. the rat orthologue of human GR alpha (mRNA and protein) was detected in caput, corpus and cauda epididymis and immunolocalized in the nucleus and cytoplasm of different epididymal cells (epithelial, smooth muscle and interstitial cells) and nerve fibers. Changes in plasma glucocorticoid and androgen levels differentially regulated GR expression in caput and cauda epididymis by homologous and heterologous mechanisms. in vivo treatment with dexamethasone significantly changed the expression of glucocorticoid-responsive genes and induced ligand-dependent GR nuclear translocation in epithelial cells from epididymis, indicating that GR is fully active in this tissue. Heterologous regulation of androgen receptor expression by glucocorticoids was also demonstrated in cauda epididymis. Our results demonstrate that the epididymis is under glucocorticoid regulation, opening new insights into the roles of this hormone in male fertility. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Keywords Rat
Glucocorticoid receptor
Gene regulation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fogarty International Center, USA
Grant number FAPESP: 05/55738-8
CNPq: 475238/2006-0
Fogarty International Center, USA: D43TW/HD00627
Fogarty International Center, USA: Unifesp/UNC 5-53284
Date 2010-08-30
Published in Molecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 325, n. 1-2, p. 64-77, 2010.
ISSN 0303-7207 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 64-77
Access rights Closed access
Type Article
Web of Science ID WOS:000280374600008

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