Analysis of Glycosaminoglycans in the Parametrium and Vaginal Apex of Women with and without Uterine Prolapse

Analysis of Glycosaminoglycans in the Parametrium and Vaginal Apex of Women with and without Uterine Prolapse

Author Kati, Lea Mina Autor UNIFESP Google Scholar
Feldner, Paulo Cezar Autor UNIFESP Google Scholar
Castro, Rodrigo Aquino de Autor UNIFESP Google Scholar
Kobayashi, Elsa Yoko Autor UNIFESP Google Scholar
Ferreira Sartori, Marair Gracio Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Batista Castello Girao, Manoel Joao Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Pelvic organ prolapse (POP) is a downward descent of pelvic organs that results in protrusions of the vagina, the uterus, or both. the cause of this disorder is likely to be multifactorial, attributable to a combination of risk factors, especially connective tissue disorders. Our objective was to characterize and quantify a component of the extracellular matrix (ECM)-sulfated glycosaminoglycan (GAG)-in the parametrium and vaginal apex of women with and without uterine prolapse.Methods: Parametrium and vaginal apex tissue was obtained from 42 women who underwent surgery. Patients underwent a physical examination and were divided into groups according to the type of genital prolapse. Standard biopsies were taken during surgery and were assessed by biochemical methods. GAGs were obtained by proteolysis. the relative concentration of GAGs was determined by densitometry. Data were compared using an independent sample t-test or chi(2) test.Results: in both groups (with and without prolapse) and in both types of tissue, dermatan sulfate (DS) was the most predominant glycosaminoglycan, followed by chondroitin sulfate (CS) and heparan sulfate (HS). We did not observe significant differences in the total amounts of GAGs, DS, CS, or HS.Conclusions: This study did not show altered biochemical characteristics in the ECM of parametrium and vaginal apex tissue of women either with or without uterine prolapse.
Language English
Sponsor Department of Gynecology and the Department of Biochemistry of the Federal University of São Paulo, São Paulo, Brazil
Date 2010-07-01
Published in Journal of Womens Health. New Rochelle: Mary Ann Liebert Inc, v. 19, n. 7, p. 1341-1344, 2010.
ISSN 1540-9996 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert Inc
Extent 1341-1344
Origin http://dx.doi.org/10.1089/jwh.2009.1567
Access rights Closed access
Type Article
Web of Science ID WOS:000279428800014
URI http://repositorio.unifesp.br/handle/11600/32693

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