Long chain saturated fatty acids increase haptoglobin gene expression in C57BL/6J mice adipose tissue and 3T3-L1 cells

Long chain saturated fatty acids increase haptoglobin gene expression in C57BL/6J mice adipose tissue and 3T3-L1 cells

Author Bueno, Allain Amador Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Motoyama, Caio Sussumu de Macedo Autor UNIFESP Google Scholar
Biz, Carolina Autor UNIFESP Google Scholar
Silveira, Vera Lucia Flor Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Institution London Metropolitan Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Dietary lipids are directly related to the composition of adipose tissue, aetiology of obesity and arousal of obesity-related pathologies, like chronic inflammation states. Haptoglobin is an acute phase protein secreted by the liver and white adipose tissue, and its blood levels vary according to the volume of fat in the body.To investigate the effect of diets enriched with large amounts of dietary fats, which differ in their fatty acid composition, on the haptoglobin gene expression by visceral and subcutaneous adipose tissue of mice fed for 2 days or 8 weeks. 3T3-L1 cells were treated with fatty acids that are found in those types of dietary fats.Mice were treated acutely (for 2 days) or chronically (for 8 weeks) with diets enriched with soybean oil, fish oil, coconut oil or lard. 3T3-L1 cells were treated with six different fatty acids. Haptoglobin gene expression was quantified by northern blotting.Both chronic and acute treatment with lard, which is rich in long chain saturated fatty acids, increased the haptoglobin mRNA expression in the retroperitoneal and epidydimal white adipose tissues. Chronic treatment with coconut oil, which is rich in medium chain saturated fatty acids, increased the haptoglobin expression in the epidydimal and subcutaneous depots. in 3T3-L1, palmitic acid increased the haptoglobin gene expression.The type of lipids in the diet can differently modulate the white adipose tissue gene expression of haptoglobin, and saturated fatty acids play a major role in promoting a pro-inflammatory environment. This response is fat pad specific and dependant on the duration of treatment.
Keywords White adipose tissue
Haptoglobin
High fat diet
Palmitic acid
Lauric acid
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-06-01
Published in European Journal of Nutrition. Heidelberg: Springer Heidelberg, v. 49, n. 4, p. 235-241, 2010.
ISSN 1436-6207 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 235-241
Origin https://dx.doi.org/10.1007/s00394-009-0069-z
Access rights Closed access
Type Article
Web of Science ID WOS:000277639500005
URI https://repositorio.unifesp.br/handle/11600/32577

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