Chronic Antioxidant Treatment Improves Arterial Renovascular Hypertension and Oxidative Stress Markers in the Kidney in Wistar Rats

Chronic Antioxidant Treatment Improves Arterial Renovascular Hypertension and Oxidative Stress Markers in the Kidney in Wistar Rats

Author Nishi, Erika Emy Autor UNIFESP Google Scholar
Oliveira-Sales, Elizabeth Barbosa Autor UNIFESP Google Scholar
Bergamaschi, Cassia T. Autor UNIFESP Google Scholar
Cesar Oliveira, Thais Galvao Autor UNIFESP Google Scholar
Boim, Mirian A. Autor UNIFESP Google Scholar
Campos, Ruy R. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract BACKGROUNDSympathetic vasomotor hyperactivity and baroreflex dysfunction are involved in the development and maintenance of renovascular arterial hypertension. We hypothesized that angiotensin (Ang) II-dependent oxidative stress contributes to the pathophysiology of the two-kidney, one-clip (2K-1C) model.METHODSThe mean arterial pressure (MAP), baroreflex, and renal sympathetic nerve activity (rSNA) were evaluated after chronic administration of an antioxidant, vitamin C (vitC 150 mg/kg/day) in male Wistar 2K-1C rats. Additionally, the mRNA levels of Ang II subtype 1 receptor (AT(1)R), NAD(P)H oxidase subunits (p47phox and gp91phox), and major antioxidant enzymes were evaluated in the renal cortex.RESULTSAfter vitC treatment, the MAP (170 +/- 4 vs. 133 +/- 6 mm Hg; P < 0.05) and rSNA (161 +/- 5 vs. 118 +/- 12 spikes/s; P < 0.05) were significantly reduced only in the 2K-1C group. VitC improved the baroreflex control of heart rate (HR) and rSNA. the expression of AT(1)R, p47phox, and gp91phox was elevated (51, 184, and 132%, respectively) in the clipped kidney of 2K-1C group. VitC downregulated AT(1)R in the clipped kidney (31%). Catalase (CAT) expression was reduced in clipped (70%) and nonclipped (83%) kidneys of 2K-1C rats. VitC treatment augmented the expression of glutathione peroxidase (GPx) in both clipped (185%) and nonclipped (212%) kidneys of the 2K-1C group.CONCLUSIONSThe present study suggests a role for oxidative stress in the cardiovascular and sympathetic alterations in renovascular hypertension, associated with changes in the expression of AT,R, NAD(P)H oxidase subunits, and antioxidant enzymes in the kidney.
Keywords baroreceptor reflex
blood pressure
oxidative stress
sympathetic nerve activity
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/15969-8
FAPESP: 06/59047-2
FAPESP: 07/56925-1
Date 2010-05-01
Published in American Journal of Hypertension. New York: Nature Publishing Group, v. 23, n. 5, p. 473-480, 2010.
ISSN 0895-7061 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent 473-480
Access rights Closed access
Type Article
Web of Science ID WOS:000277162600010

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