Study of Blood Porphyrin Spectral Profile for Diagnosis of Chronic Renal Failure

Study of Blood Porphyrin Spectral Profile for Diagnosis of Chronic Renal Failure

Author Tristao, Vivian Regina Autor UNIFESP Google Scholar
Carvalho, Fernando Felippe de Autor UNIFESP Google Scholar
Gomes, Cinthia Zanini Google Scholar
Miranda, Adriana Regina Google Scholar
Vequi-Suplicy, Cintia C. Google Scholar
Lamy, Maria Teresa Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Bellini, Maria Helena Autor UNIFESP Google Scholar
Institution IPEN CNEN SP
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption. Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. the purpose of this study was to evaluate the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eight-week-old male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated by creatinine clearance and plasma creatinine levels. the autofluorescence of erythrocytes porphyrin of healthy and NX rats was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and NX rats autofluorescence shape occurred in the 600-700 nm spectral region. A correlation was observed between emission band intensity at 635 nm and progression of renal disease.
Keywords Chronic renal failure
Language English
Date 2010-05-01
Published in Journal of Fluorescence. New York: Springer/plenum Publishers, v. 20, n. 3, p. 665-669, 2010.
ISSN 1053-0509 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 665-669
Access rights Closed access
Type Article
Web of Science ID WOS:000277937900005

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