Inducing mutations in the mouse genome with the chemical mutagen ethylnitrosourea

Inducing mutations in the mouse genome with the chemical mutagen ethylnitrosourea

Author Massironi, Sílvia Maria Gomes Google Scholar
Reis, B.l.f.s. Google Scholar
Carneiro, Juliana G. Google Scholar
Barbosa, Luciene B. S. Google Scholar
Ariza, Carolina Batista Autor UNIFESP Google Scholar
Santos, Gilmara Cristina dos Google Scholar
Guénet, Jean Louis Google Scholar
Godard, Ana Lúcia Brunialti Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de Biologia Geral
Universidade Federal de São Paulo (UNIFESP)
Institut Pasteur Département de Biologie du Développement
Abstract When compared to other model organisms whose genome is sequenced, the number of mutations identified in the mouse appears extremely reduced and this situation seriously hampers our understanding of mammalian gene function(s). Another important consequence of this shortage is that a majority of human genetic diseases still await an animal model. To improve the situation, two strategies are currently used: the first makes use of embryonic stem cells, in which one can induce knockout mutations almost at will; the second consists of a genome-wide random chemical mutagenesis, followed by screening for mutant phenotypes and subsequent identification of the genetic alteration(s). Several projects are now in progress making use of one or the other of these strategies. Here, we report an original effort where we mutagenized BALB/c males, with the mutagen ethylnitrosourea. Offspring of these males were screened for dominant mutations and a three-generation breeding protocol was set to recover recessive mutations. Eleven mutations were identified (one dominant and ten recessives). Three of these mutations are new alleles (Otop1mlh, Foxn1sepe and probably rodador) at loci where mutations have already been reported, while 4 are new and original alleles (carc, eqlb, frqz, and Sacc). This result indicates that the mouse genome, as expected, is far from being saturated with mutations. More mutations would certainly be discovered using more sophisticated phenotyping protocols. Seven of the 11 new mutant alleles induced in our experiment have been localized on the genetic map as a first step towards positional cloning.
Keywords Mouse
Ethylnitrosourea
Mutation
Co-isogenic mutations
Mutagenesis
Language English
Date 2006-09-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 39, n. 9, p. 1217-1226, 2006.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1217-1226
Origin http://dx.doi.org/10.1590/S0100-879X2006000900009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000240545900009
SciELO ID S0100-879X2006000900009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/3242

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