Storage results in loss of the antigenotoxic properties of lentinula edodes (Shiitake Mushroom) and development of in vivo genotoxicity
Lima, Patricia Lepage Alves de
Ribeiro, Daniel Araki [UNIFESP]
Sugui, Marina Mariko
Terezan, Ana Paula
Domingues, Maria Aparecida Custodio
Rodrigues Filho, Edson
Salvadori, Daisy Maria Favero
Ribeiro, Lucia Regina
Is part ofJournal of Food Biochemistry
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In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at -20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. On day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. the mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. the results indicated that storage at both room temperature and at -20C significantly reduced the antigenotoxic effects of the mushroom powder. in addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at -20C resulted in genotoxic activity (P < 0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity.
CitationJournal of Food Biochemistry. Malden: Wiley-Blackwell Publishing, Inc, v. 34, n. 1, p. 206-228, 2010.
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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