Homocysteine Thiolactone Induces Cardiac Dysfunction: Role of Oxidative Stress

Date
2010-02-01Author
Mendes, Roberta Hack
Sirvente, Raquel de Assis
Candido, Georgia Orsi
Mostarda, Cristiano
Salemi, Vera Maria Cury
D'Almeida, Vânia [UNIFESP]
Jacob, Maria Helena Vianna Metello
Ribeiro, Maria Flavia Marques
Bello-Klein, Adriane
Rigatto, Katya
Irigoyen, Maria Claudia
Type
ArtigoISSN
0160-2446Is part of
Journal of Cardiovascular PharmacologyDOI
10.1097/FJC.0b013e3181ce5c28Metadata
Show full item recordAbstract
This study investigates the cardiac functioning in male Wistar rats after treatments with methionine and homocysteine thiolactone (HcyT). the rats were distributed into 3 groups and treated for 8 weeks. Group I was the control (CO) group, given water, group II was treated with methionine, and group III with HcyT (100 mg/kg). Morphometric and functional cardiac parameters were evaluated by echocardiography. Superoxide dismutase (SOD), catalase, and glutathione S-transferase activities, chemiluminescence, thiobarbituric acid reactive substances, and immunocontent were measured in the myocardium. Hyperhomocysteinemia was observed in rats submitted to the both treatments. the results showed diastolic function was compromised in HcyT group, seen by the increase of E/A (peak velocity of early (E) and late (A) diastolic filling) ratio, decrease in deceleration time of E wave and left ventricular isovolumic relaxation time. Myocardial performance index was increased in HcyT group and was found associated with increased SOD immunocontent. HcyT group demonstrated an increase in SOD, catalase, and glutatione S-transferase activity, and chemiluminescence and thiobarbituric acid reactive substances. Overall, these results indicated that HcyT induces a cardiac dysfunction and could be associated with oxidative stress increase in the myocardium.
Citation
Journal of Cardiovascular Pharmacology. Philadelphia: Lippincott Williams & Wilkins, v. 55, n. 2, p. 198-202, 2010.Collections
- ISS - Artigos [697]