Partial protective responses induced by a recombinant cysteine proteinase from Leishmania (Leishmania) amazonensis in a murine model of cutaneous leishmaniasis
Cardoso Fedeli, Carlos Eduardo
Ferreira, Josie Haydée Lima [UNIFESP]
Mussalem, Juliana Sekeres
Longo-Maugeri, Ieda Maria
Gentil, Luciana Girotto
Machado dos Santos, Marcia Regina
Barbieri, Clara Lucia [UNIFESP]
Is part ofExperimental Parasitology
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A 500 bp fragment encoding an isoform of cysteine proteinase from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24 kDa, rLacys24. in Western blots of L (L) amazonensis extracts, antibodies directed to rLacys24 recognized a cysteine proteinase isoform of 30 kDa. Analysis by fluorescence-activated cell sorter showed a significantly higher expression of CD8(+) lymphocytes in animals immunized with rLacys24 plus CFA, whereas a low expression of CD4(+) lymphocytes was observed in these animals. the cytotoxicity of lymphocytes isolated from mice immunized with rLacys24 plus CFA on L. (L.) amazonensis-infected macrophages was significantly higher than that observed in the presence of lymphocytes from control animals. Immunization of BALB/c mice with rLacys24 plus CFA resulted in a low but significant decrease of foot lesions after challenge with L (L) amazonensis compared to those exhibited by control mice. (C) 2009 Elsevier Inc. All rights reserved.
CitationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 124, n. 2, p. 153-158, 2010.
KeywordsLeishmania (Leishmania) amazonensis
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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