Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy

Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy

Author Mitrano, Presciliana Autor UNIFESP Google Scholar
Nogueira, Marie Doki Autor UNIFESP Google Scholar
Feldner, Paulo Cezar Autor UNIFESP Google Scholar
Castro, Rodrigo Aquino Autor UNIFESP Google Scholar
Ferreira Sartori, Marair Gracio Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Batista Castello Girao, Manoel Joao Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The objective of this study is to evaluate pregnancy's action on glycosaminoglycans (GAGs) profile in bladder and urethra of female adult rats.Twenty adult female rats were divided into four groups: control, day 10 pregnancy, day 20 pregnancy, and day 5 after delivery. Sulfated GAGs content were determined by densitometry. Hyaluronic acid was assessed by fluorimetric enzyme-linked immunosorbent serologic-like assay. Radioactive GAGs were quantified by measuring radioactivity in electrophoresis gel. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney tests.In all groups, dermatan sulfate was predominant followed by heparan sulfate and hyaluronic acid. Significant decrease in total GAGs in bladder was observed at the beginning of pregnancy, with progressive increase. Biosynthesis of such molecules showed a similar behavior. in urethra, changes in GAGs content were restricted to the end of pregnancy. Hyaluronic acid content showed a significant increase in bladder during puerperium.We observed different composition in vesical and urethral tissues during pregnancy and in puerperium.
Keywords Extracellular matrix
Language English
Date 2010-02-01
Published in International Urogynecology Journal. Artington: Springer London Ltd, v. 21, n. 2, p. 241-246, 2010.
ISSN 0937-3462 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 241-246
Access rights Closed access
Type Article
Web of Science ID WOS:000273787500017

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