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dc.contributor.authorCosta, Robson
dc.contributor.authorManjavachi, Marianne N.
dc.contributor.authorMotta, Emerson M.
dc.contributor.authorMarotta, Denise M.
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorTorres, Hugo Arruda de Moura [UNIFESP]
dc.contributor.authorPesquero, João Bosco [UNIFESP]
dc.contributor.authorCalixto, Joao B.
dc.identifier.citationBritish Journal of Pharmacology. Malden: Wiley-Blackwell, v. 159, n. 4, p. 888-897, 2010.
dc.description.abstractBackground and purpose:Activation of the proteinase-activated receptor-2 (PAR-2) induces scratching behaviour in mice. Here, we have investigated the role of kinin B-1 and B-2 receptors in the pruritogenic response elicited by activators of PAR-2.Experimental approach:Scratching was induced by an intradermal (i.d.) injection of trypsin or the selective PAR-2 activating peptide SLIGRL-NH2 at the back of the mouse neck. the animals were observed for 40 min and their scratching response was quantified.Key results:I.d. injection of trypsin or SLIGRL-NH2 evoked a scratching behaviour, dependent on PAR-2 activation. Mice genetically deficient in kinin B-1 or B-2 receptors exhibited reduced scratching behaviour after i.d. injection of trypsin or SLIGRL-NH2. Treatment (i.p.) with the non-peptide B-1 or B-2 receptor antagonists SSR240612 and FR173657, respectively, prevented the scratching behaviour caused by trypsin or SLIGRL-NH2. Nonetheless, only treatment i.p. with the peptide B-2 receptor antagonist, Hoe 140, but not the B-1 receptor antagonist (DALBK), inhibited the pruritogenic response to trypsin. Hoe 140 was also effective against SLIGRL-NH2-induced scratching behaviour when injected by i.d. or intrathecal (i.t.) routes. Also, the response to SLIGRL-NH2 was inhibited by i.t. (but not by i.d.) treatment with DALBK. Conversely, neither Hoe 140 nor DALBK were able to inhibit SLIGRL-NH2-induced scratching behaviour when given intracerebroventricularly (i.c.v.).Conclusions and implications:The present results demonstrated that kinins acting on both B-1 and B-2 receptors played a crucial role in controlling the pruriceptive signalling triggered by PAR-2 activation in mice.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipPrograma de Apoio aos Nucleos de Excelencia (PRONEX)
dc.description.sponsorshipFundacao de Apoio a Pesquisa do Estado de Santa Catarina (FAPESC)
dc.relation.ispartofBritish Journal of Pharmacology
dc.rightsAcesso aberto
dc.subjectscratching behaviouren
dc.subjectkinin B-2 and B-1 receptorsen
dc.titleThe role of kinin B-1 and B-2 receptors in the scratching behaviour induced by proteinase-activated receptor-2 agonists in miceen
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv Fed Santa Catarina, Dept Pharmacol, Ctr Biol Sci, BR-88049900 Florianopolis, SC, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.sourceWeb of Science

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