Immobilized Kidney 28-kDa Endostatin- Related (KES28kDa) Fragment Promotes Endothelial Cell Survival

Immobilized Kidney 28-kDa Endostatin- Related (KES28kDa) Fragment Promotes Endothelial Cell Survival

Author Bellini, Maria Helena Autor UNIFESP Google Scholar
Malpighi, Thiago Franca Google Scholar
Calvo, Fernanda Bernardes Google Scholar
Miranda, Adriana Regina Google Scholar
Spencer, Patrick Jack Google Scholar
Cichy, Milena Cristina Autor UNIFESP Google Scholar
Simons, Simone Michaela Google Scholar
Chudzinski Tavassi, Ana Marisa Google Scholar
Santos, Marinilce Fagundes dos Google Scholar
Rodrigues, Consuelo Junqueira Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Institution CNEN SP
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background/Objective: Renal ischemia-hypoxia is a leading cause of acute kidney injury (AKI). Ischemia causes extracellular matrix breakdown of the tubular basement membrane. Endostatin (ES) is the C-terminal fragment of collagen XVIII generated by proteolytic cleavage. Recent studies have demonstrated that ES expression is upregulated in ischemic kidneys. the present study aimed to characterize ES from ischemic kidneys. Methods: Ischemic renal failure was induced via 45 min of occlusion of the left renal artery and vein. After the ischemic period, blood was collected. Kidneys were harvested and used for immunohistochemical testing and protein extraction. Three-step purification was used. Soluble and immobilized purified ES were tested in cell viability and adhesion assays. Results: the soluble KES28kDa inhibited endothelial cell proliferation: 25 versus 12.5 mu g (p < 0.05); 12.5 versus 3.15 mu g (p < 0.05). Immobilization of KES28kDa supports endothelial cell survival over the control p = 0.021). Human umbilical vein endothelial cells plated on immobilized KES28kDa showed an increase in membrane ruffles and stress fibers. Conclusion: These data demonstrate the local synthesis of a 28-kDa ES-related fragment following AKI and suggest its role in endothelium survival. Copyright (C) 2010 S. Karger AG, Basel
Keywords Acute kidney injury
Endothelial cell survival
Human umbilical vein endothelial cells
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2007-51204-4
Date 2010-01-01
Published in American Journal of Nephrology. Basel: Karger, v. 31, n. 3, p. 255-261, 2010.
ISSN 0250-8095 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 255-261
Access rights Closed access
Type Article
Web of Science ID WOS:000275678400009

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