Ischemia/reperfusion is an independent trigger for increasing myocardial content of mRNA B-type natriuretic peptide

Ischemia/reperfusion is an independent trigger for increasing myocardial content of mRNA B-type natriuretic peptide

Author Ramos, Lafayete William F. Autor UNIFESP Google Scholar
Murad, Neif Autor UNIFESP Google Scholar
Goto, Eduardo Autor UNIFESP Google Scholar
Antonio, Edinei L. Autor UNIFESP Google Scholar
Silva, Jose A. Google Scholar
Tucci, Paulo F. Autor UNIFESP Google Scholar
Carvalho, Antonio C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Ctr Nove Julho
Abstract This study aims to determine whether a relation exists between ischemia/reperfusion and myocardial B-type natriuretic peptide (BNP) mRNA expression independent of variations in intracavitary diastolic volume and consequently, of cardiomyocyte stretching. Twenty-three rats were subjected to the following conditions: control (C), 15 min of ischemia (I15), or ischemia plus 15 (R15), 30 (R30), or 45 (R45) min of reperfusion in the in situ hearts. Isolated hearts of sixteen additional rats (sham, n = 8; occlusion, n = 8) were perfused for studies in the absence of ventricular distension. All hearts were divided in two segments (ischemic and nonischemic). Ventricular distension was avoided by excluding the atria and mitral valves. in both experiments, BNP mRNA was quantified by real-time polymerase chain reaction in both nonischemic and ischemic regions. in the in situ hearts, myocardial BNP mRNA values at R15 (4.24 +/- 0.75) in the ischemic region were higher than in other groups (C: 1.43 +/- 0.81, P = 0.044; I15: 3.05 +/- 0.62, P = 0.048; R30: 0.76 +/- 0.84, P = 0.001; R45: 1.47 +/- 0.60, P = 0.046, [analysis of variance]). in isolated hearts without ventricular distension, myocardial BNP mRNA (arbitrary units) content at R15 in ischemic regions (4.54 +/- 0.26) was greater than in nonischemic regions in both occlusion (3.51 +/- 0.20, P < 0.001) and sham (3.38 +/- 0.25, P = 0.0001 and 3.47 +/- 0.19, P = 0.0001) groups. the present data show that ischemia/reperfusion is responsible for increased BNP mRNA myocardial content independent of changes of ventricular cavity diastolic volume.
Keywords Ischemia/reperfusion
B-type natriuretic peptide
Coronary artery disease
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-11-01
Published in Heart and Vessels. New York: Springer, v. 24, n. 6, p. 454-459, 2009.
ISSN 0910-8327 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 454-459
Access rights Closed access
Type Article
Web of Science ID WOS:000272073400011

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