Atypical enteropathogenic Escherichia coli genomic background allows the acquisition of non-EPEC virulence factors

Date
2009-10-01Author
Bando, Silvia Y.
Andrade, Fernanda B.
Guth, Beatriz E. C. [UNIFESP]
Elias Junior, Waldir Pereira [UNIFESP]
Moreira-Filho, Carlos A.
Pestana de Castro, Antonio F.
Type
ArtigoISSN
0378-1097Is part of
Fems Microbiology LettersDOI
10.1111/j.1574-6968.2009.01735.xMetadata
Show full item recordAbstract
Atypical enteropathogenic Escherichia coli (aEPEC) has been associated with infantile diarrhea in many countries. the clonal structure of aEPEC is the object of active investigation but few works have dealt with its genetic relationship with other diarrheagenic E. coli (DEC). This study aimed to evaluate the genetic relationship of aEPEC with other DEC pathotypes. the phylogenetic relationships of DEC strains were evaluated by multilocus sequence typing. Genetic diversity was assessed by pulsed-field gel electrophoresis (PFGE). the phylogram showed that aEPEC strains were distributed in four major phylogenetic groups (A, B1, B2 and D). Cluster I ( group B1) contains the majority of the strains and other pathotypes [enteroaggregative, enterotoxigenic and enterohemorrhagic E. coli ( EHEC)]; cluster II ( group A) also contains enteroaggregative and diffusely adherent E. coli; cluster III ( group B2) has atypical and typical EPEC possessing H6 or H34 antigen; and cluster IV ( group D) contains aEPEC O55:H7 strains and EHEC O157:H7 strains. PFGE analysis confirmed that these strains encompass a great genetic diversity. These results indicate that aEPEC clonal groups have a particular genomic background - especially the strains of phylogenetic group B1 that probably made possible the acquisition and expression of virulence factors derived from non-EPEC pathotypes.
Citation
Fems Microbiology Letters. Malden: Wiley-Blackwell Publishing, Inc, v. 299, n. 1, p. 22-30, 2009.Keywords
atypical enteropathogenic Escherichia colidiarrheagenic Escherichia coli
multilocus sequence typing
pulsed-field gel electrophoresis
Sponsorship
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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- EPM - Artigos [17701]