Ang-(3-4) suppresses inhibition of renal plasma membrane calcium pump by Ang II

Ang-(3-4) suppresses inhibition of renal plasma membrane calcium pump by Ang II

Author Axelband, Flavia Google Scholar
Assuncao-Miranda, Iranaia Google Scholar
Paula, Isabela R. de Google Scholar
Ferrao, Fernanda M. Google Scholar
Dias, Juliana Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Miranda, Filipe Google Scholar
Lara, Lucienne S. Google Scholar
Vieyra, Adalberto Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract We previously demonstrated that Ang II inhibits the renal plasma membrane Ca(2+)-ATPase. in the present work we have studied the effect of Ang II, at concentrations similar to those found in the renal interstitium, on the Ca(2+)-ATPase from proximal tubule cells. High Ang II concentration (5 x 10(-7) mol/L) led to the recovery of Ca(2+)-ATPase activity previously inhibited by 50% at low Ang II concentration (10(-10) mol/L). Reacdvation occurred in parallel with: (i) formation of only two dead-end metabolites [Ang-(3-4) and Tyr] after incubation of isolated membranes with micromolar Ang II; and (ii) dissociation of constitutive AT(1)R/AT(2)R heterodimers, which are preserved with 10(-10) mol/LAng II. When the membranes were incubated with 10(-14) mol/LAng-(3-4), inhibition by 10(-10) mol/L Ang II was no longer observed. the counteracting effect of Ang-(3-4) was abolished by PD123319, an antagonist of AT(2)R, and mimicked by CGP42112A, an agonist of AT(2)R. Ang-(1-7) is an intermediate in the formation of Ang(3-4) via a pathway involving angiotensin-converting enzyme (ACE), and complete dipeptide breakdown to Tyr and Val is impaired by low Ang II. We conclude that Ang-(3-4) may be a physiological regulator of active Ca(2+) fluxes in renal proximal cells by acting within the renin-angiotensin axis. (C) 2009 Elsevier B.V. All rights reserved.
Keywords Renin-angiotensin system
Angiotensin receptors
Active calcium transport
Kidney cells
Basolateral membranes
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-06-05
Published in Regulatory Peptides. Amsterdam: Elsevier B.V., v. 155, n. 1-3, p. 81-90, 2009.
ISSN 0167-0115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 81-90
Access rights Closed access
Type Article
Web of Science ID WOS:000267458200014

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