Validation of commonly used reference genes for sleep-related gene expression studies

View/ Open
Date
2009-05-15Author
Lee, Kil Sun [UNIFESP]
Alvarenga, Tathiana Aparecida [UNIFESP]
Guindalini, Camila [UNIFESP]
Andersen, Monica Levy [UNIFESP]
Castro, Rosa Maria Rodrigues Pinto Santos [UNIFESP]
Tufik, Sergio [UNIFESP]
Type
ArtigoISSN
1471-2199Is part of
Bmc Molecular BiologyDOI
10.1186/1471-2199-10-45Metadata
Show full item recordAbstract
Background: Sleep is a restorative process and is essential for maintenance of mental and physical health. in an attempt to understand the complexity of sleep, multidisciplinary strategies, including genetic approaches, have been applied to sleep research. Although quantitative real time PCR has been used in previous sleep-related gene expression studies, proper validation of reference genes is currently lacking. Thus, we examined the effect of total or paradoxical sleep deprivation (TSD or PSD) on the expression stability of the following frequently used reference genes in brain and blood: beta-actin (b-actin), beta-2-microglobulin (B2M), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine guanine phosphoribosyl transferase (HPRT).Results: Neither TSD nor PSD affected the expression stability of all tested genes in both tissues indicating that b-actin, B2M, GAPDH and HPRT are appropriate reference genes for the sleep-related gene expression studies. in order to further verify these results, the relative expression of brain derived neurotrophic factor (BDNF) and glycerol-3-phosphate dehydrogenase1 (GPD1) was evaluated in brain and blood, respectively. the normalization with each of four reference genes produced similar pattern of expression in control and sleep deprived rats, but subtle differences in the magnitude of expression fold change were observed which might affect the statistical significance.Conclusion: This study demonstrated that sleep deprivation does not alter the expression stability of commonly used reference genes in brain and blood. Nonetheless, the use of multiple reference genes in quantitative RT-PCR is required for the accurate results.
Citation
Bmc Molecular Biology. London: Biomed Central Ltd, v. 10, 8 p., 2009.Sponsorship
Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
AFIP
Collections
- EPM - Artigos [17677]
Related items
Showing items related by title, author, creator and subject.
-
I Diretriz brasileira de cardio-oncologia pediátrica da Sociedade Brasileira de Cardiologia
Seber, Adriana [UNIFESP]; Miachon, Adriana Siviero [UNIFESP]; Tanaka, Ana Cristina Sayuri; Spinola-Castro, Angela Maria [UNIFESP]; Carvalho, Antonio Carlos [UNIFESP]; Petrilli, Antonio Sergio [UNIFESP]; Macedo, Carla Renata Pacheco Donato [UNIFESP]; Nars, Clarissa Carvalho Fongaro [UNIFESP]; Terzian, Claudia Naufel [UNIFESP]; Castro Junior, Claudio Galvão de; Santos, Cleusa Cavalcanti Lapa; Guerra, Cristina Chaves dos Santos; Silva, Dafne Cardoso Bourguignon da [UNIFESP]; Bassi, Débora Ugayama; Azeka, Estela; Feitosa, Fabiana Aragão; Hamamoto, Fernando [UNIFESP]; Szarf, Gilberto [UNIFESP]; Lederman, Henrique Manoel [UNIFESP]; Rigon Junior, Humberto João; Jatene, Ieda Biscegli; Mota, Isabele Coelho Fonseca da; Perrud, Jeferson Adriano [UNIFESP]; Soares Junior, José; Gutierrez, Julián Arango; Perin, Juliana Pepe Marinho [UNIFESP]; Soares, Juliana dos Santos [UNIFESP]; Catani, Liane Hulle; Tsai, Liliana Yu [UNIFESP]; Vianna, Livia Cristina [UNIFESP]; Paiva, Marcelo Goulart; Santos, Marcelo José; Ishigai, Marcia Marcelino de Souza [UNIFESP]; Diógenes, Maria Suely Bezerra [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Piedade, Maria Tereza Castro [UNIFESP]; Parreiras, Mariana; Cypriano, Mônica [UNIFESP]; Negrini, Nilce da Silva [UNIFESP]; Campos Filho, Orlando [UNIFESP]; Figueiredo, Paula Andrade [UNIFESP]; Novaes, Paulo Eduardo [UNIFESP]; Camargo, Paulo Roberto [UNIFESP]; Maia, Priscila dos Santos [UNIFESP]; Petrilli, Renata [UNIFESP]; Arduini, Rodrigo Genaro [UNIFESP]; Gouveia, Roseane Vasconcelos [UNIFESP]; Teruya, Suzana Barbosa Miranda [UNIFESP]; Moisés, Valdir Ambrósio [UNIFESP]; Morais, Vera Lúcia Lins de (Sociedade Brasileira de Cardiologia - SBC, 2013-05-01) -
Guerras culturais em verde e amarelo
Arantes, Pedro Fiori [UNIFESP]; Okuma, André [UNIFESP]; Nascimento, Giulia dos Santos [UNIFESP]; Melo, Keyla Vasconcelos de [UNIFESP]; Lorena, Diego [UNIFESP]; Alves, Amanda [UNIFESP]; Brait, Ana Laura [UNIFESP]; Alves, Gustavo Almeida [UNIFESP]; Fernandes, Cristina Naiara [UNIFESP]; Avelino, Larissa [UNIFESP]; Paloma, Oliveira [UNIFESP]; Bertellotti, André Zaparoli [UNIFESP]; Carreño, Rebeca Nieves Inostroza [UNIFESP]; Feo, Amanda [UNIFESP]; Andrade, Bruna [UNIFESP]; Garotti, Josiane [UNIFESP]; Flauto, Larissa [UNIFESP]; Tavares, Melissa [UNIFESP]; Barbosa, Ana Beatriz Tavares [UNIFESP]; Alejarra, Melissa Maria dos Santos [UNIFESP]; Santos, Nicole Pinheiro [UNIFESP]; Dourado, Kamilla [UNIFESP]; Silva, Pamela [UNIFESP]; Goyano, Lucius [UNIFESP]; Meneses, Maria Luiza [UNIFESP]; Ferreira, Gabriela [UNIFESP]; Monteiro, Paloma [UNIFESP]; Oliveira, Marcelo Lauton de [UNIFESP]; Paula, Patrícia Pinheiro Antunes de [UNIFESP]; Santos, Isabella Mendes dos [UNIFESP]; Borges, Júlia Rodrigues [UNIFESP] (Universidade Federal de São Paulo, 2022)Queridos/as leitores/as, bem-vindos/as ao nosso campo de batalha – e de estudos. Vocês estão prestes a ler um livro perigoso. Ele fala sobre muito do que estamos vivendo no Brasil dos últimos anos, olhando esse nosso ...
-
Experience Report on Setting Up a Transdisciplinary Outpatient Unit for Transgender Health
Vieira, Denise Leite [UNIFESP]; Fanganiello, Ana Luiza [UNIFESP]; Zaneti, Marina Milograna [UNIFESP]; Vieira, Fernanda de Sousa [UNIFESP]; Brasolin, Adriano [UNIFESP]; Rocha, Natalia Turena [UNIFESP]; Souza, Eduardo Sodré de [UNIFESP]; Silveira, Mariana [UNIFESP]; Cervi, Thales [UNIFESP]; Penteado, Sonia [UNIFESP]; Brito, Maria Jose [UNIFESP]; Alves, Juliana [UNIFESP]; Mota, Marisa [UNIFESP]; Holanda, Fabiola [UNIFESP]; Tanaka, Luiza [UNIFESP]; Fernandes, Maria Jose [UNIFESP]; Takano, Claudia [UNIFESP]; Yarak, Samira [UNIFESP]; Azevedo, Renata [UNIFESP]; Gois, Aecio [UNIFESP]; Silva, Ivaldo [UNIFESP]; Mari, Jair [UNIFESP]; Pereira, Pedro [UNIFESP]; Ferreira, Lydia [UNIFESP]; Dias-Da-Silva, Magnus R. [UNIFESP]; Sodre, Eduardo [UNIFESP] (Wiley, 2017)