Galanin Is a Selective Marker of the Retrotrapezoid Nucleus in Rats
Stornetta, Ruth L.
Moreira, Thiago S. [UNIFESP]
Takakura, Ana C. [UNIFESP]
West, Gavin H.
Gwilt, Justin M.
Pilowsky, Paul M.
Guyenet, Patrice G.
Is part ofJournal of Comparative Neurology
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The rat retrotrapezoid nucleus (RTN) contains CO(2)-activated neurons that contribute to the central chemoreflex and to breathing automaticity. These neurons have two known markers, the transcription factor Phox2b and vesicular glutamate transporter 2 (VGLUT2). Noncatecholaminergic galanin-immunoreactive (ir) neurons within a region of the lower brainstem that seems identical to what is currently defined as the RTN have been previously described. Here we ask whether these galanin-expressing neurons are the same cells as the recently characterized CO(2)-sensitive neurons of the RTN. By using in situ hybridization, we found that pre-pro-galanin (PPGal) mRNA is expressed by an isolated cluster of neurons that is co-extensive with the RTN as defined by a population of strongly Phox2b-ir neurons devoid of tyrosine hydroxylase (Phox2b(+)/TH(-) neurons). This bilateral structure contains about 1,000 PPGal mRNA-positive neurons in the rat. the IPPGal mRNA-positive neurons were Phox2b(+)/TH(-) and as susceptible to destruction by the toxin [Sar(9), Met (O(2))(11)]-substance P as the rest of the RTN Phox2b(+)/TH(-) cells of the RTN. CO(2)-activated neurons were recorded in the RTN of anesthetized rats and were labeled with biotinamide. Many of those cells (7/17, 41%, five rats) contained PPGal-mRNA. in conclusion, galanin mRNA is a very specific marker of the glutamatergic Phox2b(+)/TH(-) neurons of the RTN, but galanin mRNA identifies only half of these putative central respiratory chemoreceptors. J. Comp. Neurol. 512:373-383, 2009. (C) 2008 Wiley-Liss, Inc.
CitationJournal of Comparative Neurology. Hoboken: Wiley-liss, v. 512, n. 3, p. 373-383, 2009.
SponsorshipNational Institutes of Health
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