Vital Dyes and Light Sources for Chromovitrectomy: Comparative Assessment of Osmolarity, pH, and Spectrophotometry

Vital Dyes and Light Sources for Chromovitrectomy: Comparative Assessment of Osmolarity, pH, and Spectrophotometry

Author Costa, Elaine de Paula Fiod Autor UNIFESP Google Scholar
Rodrigues, Eduardo Buchele Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Penha, Fernando Marcondes Autor UNIFESP Google Scholar
Magalhaes, Octaviano Autor UNIFESP Google Scholar
Furlani, Bruno Albuquerque Autor UNIFESP Google Scholar
Lima Filho, Acácio Alves de Souza Autor UNIFESP Google Scholar
Miranda, Antonio de Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE. To investigate the in vitro pH, osmolarity, spectral, and photostability properties of nine vital dyes for vitreoretinal surgery.METHODS. Nine dyes-indocyanine green (ICG), trypan blue (TB), brilliant blue (BriB), bromophenol blue (BroB), Congo red (CR), light green (LG), fast green (FG), indigo carmine (IC) and Evans blue (EB)-diluted in three solvents (saline solution, glucose 5%, and water) were tested for osmolarity and pH. Spectrophotometry was used to determine absorbance properties of 27 solutions. Irradiance emission spectra of seven endoillumination light sources and fiber-optics were compared with dye absorbance curves.RESULTS. Dye osmolarity in saline solution and glucose 5% varied widely (257-385 mOsm) and was lower (0-54 mOsm) when dyes were dissolved in water. Dyes diluted in three solvents showed pH values varying from 2.6 to 9.85. ICG, LG, TB, BroB, CR, and IC demonstrated different absorbances, depending on the solvent. BriB and FG showed similar absorbance curves with different solvents. Spectrophometric analysis showed that all dyes except ICG had remarkable spectral overlap with the light sources. Among endoillumination fiber-optics, overlap was greatest with dual-output illumination with an integrated laser pathway and least with a mercury vapor lamp.CONCLUSIONS. Vital dyes showed variable osmolarity and pH, which also depended on the solvent used. Interaction of light from endoillumination source and vital dye may increase or decrease the risk for toxicity, making appropriate selection of both a desirable way to minimize the risk for phototoxic effects. (Invest Ophthalmol Vis Sci. 2009; 50: 385-391) DOI: 10.1167/iovs.08-2285
Language English
Date 2009-01-01
Published in Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 50, n. 1, p. 385-391, 2009.
ISSN 0146-0404 (Sherpa/Romeo, impact factor)
Publisher Assoc Research Vision Ophthalmology Inc
Extent 385-391
Origin http://dx.doi.org/10.1167/iovs.08-2285
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000262199900050
URI http://repositorio.unifesp.br/handle/11600/31119

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