Co-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitro

Co-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitro

Author Popi, Ana F. Autor UNIFESP Google Scholar
Motta, Fabiana L. T. Autor UNIFESP Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Lopes, Jose D. Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We previously demonstrated that B-1b cells can undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to substrate in vitro. Here we followed the expression of surface markers and transcription factors during this differentiation. B-1b cells spontaneously express both myeloid and lymphoid restricted transcription factors. When induced to differentiate into a phagocyte, the lymphoid genes E box protein (E2A), early B-cell factor (EBF), paired box 5 (Pax5) are down-modulated, while expression of genes related to myeloid commitment is sustained. Furthermore, B-1b cell-derived phagocytes (B-1CDPs) decrease immunoglobulin M (IgM) expression but retain the expression of the heavy chain variable gene VH11 or VH12, an immunoglobulin gene rearrangement predominantly expressed by B-1 cells. the maintenance of lymphoid characteristics in B-1CDPs characterizes a unique type of phagocyte, not related to monocyte-derived macrophages.
Keywords B-1 cells
differentiation
macrophage
phagocytosis
transcription factors
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/14837-0
FAPESP: 04/08506-1
Date 2009-01-01
Published in Immunology. Malden: Wiley-Blackwell Publishing, Inc, v. 126, n. 1, p. 114-122, 2009.
ISSN 0019-2805 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 114-122
Origin http://dx.doi.org/10.1111/j.1365-2567.2008.02883.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000261528300013
URI http://repositorio.unifesp.br/handle/11600/31110

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