Modulation of the type I hypersensitivity late phase reaction to OVA by Propionibacterium acnes-soluble polysaccharide

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dc.contributor.author Squaiella, Carla Cristina [UNIFESP]
dc.contributor.author Figueiredo Longhini, Ana Leda [UNIFESP]
dc.contributor.author Braga, Eleni Goncalves [UNIFESP]
dc.contributor.author Mussalem, Juliana Sekeres [UNIFESP]
dc.contributor.author Ananias, Renata Zeigler [UNIFESP]
dc.contributor.author Yendo, Tatiana Mina [UNIFESP]
dc.contributor.author Straus, Anita H. [UNIFESP]
dc.contributor.author Toledo, Marcos S. [UNIFESP]
dc.contributor.author Takahashi, Helio K. [UNIFESP]
dc.contributor.author Hirata, Izaura Yoshico [UNIFESP]
dc.contributor.author Longo-Maugeri, Ieda Maria [UNIFESP]
dc.date.accessioned 2016-01-24T13:51:59Z
dc.date.available 2016-01-24T13:51:59Z
dc.date.issued 2008-12-22
dc.identifier http://dx.doi.org/10.1016/j.imlet.2008.10.005
dc.identifier.citation Immunology Letters. Amsterdam: Elsevier B.V., v. 121, n. 2, p. 157-166, 2008.
dc.identifier.issn 0165-2478
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31104
dc.description.abstract Late phase reaction (LPR) of immediate hypersensitivity is a Th2 response characterized by eosinophil recruitment and related to allergic asthma pathogenesis. Several strategies were developed trying to control the tissue damage observed in this reaction. Recently, we verified that killed Propionibacterium acnes (P. acnes), a Gram-positive bacillus, immunomodulated LPR in a murine model, potentiating or suppressing it depending on the treatment protocol used. However, the bacterium compounds responsible for this effect are not known, leading us to investigate if P acnes purified Soluble polysaccharide (PS) could be a major component involved on the modulation induced by the bacterium. Recently. we demonstrated that PS, like P. acnes, induces adjuvant effect on DNA vaccine, increases bone marrow dendritic cell precursors in vivo and its maturation in vitro, and modulates in vitro macrophage tumoricidal activity. Herein, we determined the chemical PS composition, which is mainly constituted by galactopyranose, ribopyranose, arabinopyranose, glucopyranose, ribofuranose and mannopyranose, and analyzed its capacity to modulate the immediate hypersensitivity in mice. Animals were subcutaneously implanted with coagulated hen's egg white (HEW) and 14 days later challenged with ovalbumin (OVA) in the footpad, developing a typical LPR after 24 h. Similarly to the whole bacterium, Th2 response to OVA was potentiated when PS was administered concomitantly to HEW implantation, by increase in footpad eosinophilia and IL4-producing spleen cells, and decrease in anti-OVA IgG2a titers and IL-12- or IFN-gamma-producing cells. On the other hand, the reaction was abrogated when HEW implantation was performed 1 week after PS-treatment, by decrease in footpad swelling, eosinophilia and anti-OVA IgG1 levels, and increase in IgG2a titers and IL-12-producing cells, These data suggest that PS seems to be the major P. acnes compound responsible for its effects on the modulation of immediate hypersensitivity reaction in mice. (C) 2008 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 157-166
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Immunology Letters
dc.rights Acesso restrito
dc.subject Immediate hypersensitivity en
dc.subject Immunomodulation en
dc.subject Polysaccharide en
dc.subject Propionibacterium acnes en
dc.subject Adjuvant en
dc.title Modulation of the type I hypersensitivity late phase reaction to OVA by Propionibacterium acnes-soluble polysaccharide en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Disciplina Imunol, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Disciplina Imunol, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1016/j.imlet.2008.10.005
dc.description.source Web of Science
dc.identifier.wos WOS:000262769300012



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