Modulation of the type I hypersensitivity late phase reaction to OVA by Propionibacterium acnes-soluble polysaccharide

Modulation of the type I hypersensitivity late phase reaction to OVA by Propionibacterium acnes-soluble polysaccharide

Author Squaiella, Carla Cristina Autor UNIFESP Google Scholar
Figueiredo Longhini, Ana Leda Autor UNIFESP Google Scholar
Braga, Eleni Goncalves Autor UNIFESP Google Scholar
Mussalem, Juliana Sekeres Autor UNIFESP Google Scholar
Ananias, Renata Zeigler Autor UNIFESP Google Scholar
Yendo, Tatiana Mina Autor UNIFESP Google Scholar
Straus, Anita H. Autor UNIFESP Google Scholar
Toledo, Marcos S. Autor UNIFESP Google Scholar
Takahashi, Helio K. Autor UNIFESP Google Scholar
Hirata, Izaura Yoshico Autor UNIFESP Google Scholar
Longo-Maugeri, Ieda Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Late phase reaction (LPR) of immediate hypersensitivity is a Th2 response characterized by eosinophil recruitment and related to allergic asthma pathogenesis. Several strategies were developed trying to control the tissue damage observed in this reaction. Recently, we verified that killed Propionibacterium acnes (P. acnes), a Gram-positive bacillus, immunomodulated LPR in a murine model, potentiating or suppressing it depending on the treatment protocol used. However, the bacterium compounds responsible for this effect are not known, leading us to investigate if P acnes purified Soluble polysaccharide (PS) could be a major component involved on the modulation induced by the bacterium. Recently. we demonstrated that PS, like P. acnes, induces adjuvant effect on DNA vaccine, increases bone marrow dendritic cell precursors in vivo and its maturation in vitro, and modulates in vitro macrophage tumoricidal activity. Herein, we determined the chemical PS composition, which is mainly constituted by galactopyranose, ribopyranose, arabinopyranose, glucopyranose, ribofuranose and mannopyranose, and analyzed its capacity to modulate the immediate hypersensitivity in mice. Animals were subcutaneously implanted with coagulated hen's egg white (HEW) and 14 days later challenged with ovalbumin (OVA) in the footpad, developing a typical LPR after 24 h. Similarly to the whole bacterium, Th2 response to OVA was potentiated when PS was administered concomitantly to HEW implantation, by increase in footpad eosinophilia and IL4-producing spleen cells, and decrease in anti-OVA IgG2a titers and IL-12- or IFN-gamma-producing cells. On the other hand, the reaction was abrogated when HEW implantation was performed 1 week after PS-treatment, by decrease in footpad swelling, eosinophilia and anti-OVA IgG1 levels, and increase in IgG2a titers and IL-12-producing cells, These data suggest that PS seems to be the major P. acnes compound responsible for its effects on the modulation of immediate hypersensitivity reaction in mice. (C) 2008 Elsevier B.V. All rights reserved.
Keywords Immediate hypersensitivity
Propionibacterium acnes
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2008-12-22
Published in Immunology Letters. Amsterdam: Elsevier B.V., v. 121, n. 2, p. 157-166, 2008.
ISSN 0165-2478 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 157-166
Access rights Closed access
Type Article
Web of Science ID WOS:000262769300012

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