Collecting duct renin is upregulated in both kidneys of 2-kidney, 1-clip Goldblatt hypertensive rats

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dc.contributor.author Prieto-Carrasquero, Minolfa C.
dc.contributor.author Botros, Fady T.
dc.contributor.author Pagan, Javier
dc.contributor.author Kobori, Hiroyuki
dc.contributor.author Seth, Dale M.
dc.contributor.author Casarini, Dulce Elena [UNIFESP]
dc.contributor.author Navar, L. Gabriel
dc.date.accessioned 2016-01-24T13:51:24Z
dc.date.available 2016-01-24T13:51:24Z
dc.date.issued 2008-06-01
dc.identifier http://dx.doi.org/10.1161/HYPERTENSIONAHA.108.110916
dc.identifier.citation Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 51, n. 6, p. 1590-1596, 2008.
dc.identifier.issn 0194-911X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/30679
dc.description.abstract Renin in collecting duct cells is upregulated in chronic angiotensin II-infused rats via angiotensin II type 1 receptors. To determine whether stimulation of collecting duct renin is a blood pressure-dependent effect; changes in collecting duct renin and associated parameters were assessed in both kidneys of 2-kidney, 1-clip Goldblatt hypertensive (2K1C) rats. Renal medullary tissues were used to avoid the contribution of renin from juxtaglomerular cells. Systolic blood pressure increased to 184 +/- 9 mm Hg in 2K1C rats (n=19) compared with sham rats (121 +/- 6 mm Hg; n=12). Although renin immunoreactivity markedly decreased in juxtaglomerular cells of nonclipped kidneys (NCK: 0.2 +/- 0.0 versus 1.0 +/- 0.0 relative ratio) and was augmented in clipped kidneys (CK: 1.7 +/- 1.0 versus 1.0 +/- 0.0 relative ratio), its immunoreactivity increased in cortical and medullary collecting ducts of both kidneys of 2K1C rats (CK: 2.8 +/- 1.0 cortex; 2.1 +/- 1.0 medulla; NCK: 4.6 +/- 2.0 cortex, 3.2 +/- 1.0 medulla versus 1.0 +/- 0.0 in sham kidneys). Renal medullary tissues of 2K1C rats showed greater levels of renin protein (CK: 1.4 +/- 0.2; NCK: 1.5 +/- 0.3), renin mRNA (CK: 5.8 +/- 2.0; NCK: 4.9 +/- 2.0), angiotensin I (CK: 120 +/- 18 pg/g; NCK: 129 +/- 13 pg/g versus sham: 67 +/- 6 pg/g), angiotensin II (CK: 150 +/- 32 pg/g; NCK: 123 +/- 21 pg/g versus sham: 91 +/- 12 pg/g; P < 0.05), and renin activity (CK: 8.6 mu g of angiotensin I per microgram of protein; NCK: 8.3 mu g of angiotensin I per microgram of protein; sham: 3.4 mu g of angiotensin I per microgram of protein) than sham rats. These data indicate that enhanced collecting duct renin in 2K1C rats occurs independently of blood pressure. Upregulation of distal tubular renin helps to explain how sustained intrarenal angiotensin II formation occurs even during juxtaglomerular renin suppression, thus allowing maintained effects on tubular sodium reabsorption that contribute to the hypertension. en
dc.format.extent 1590-1596
dc.language.iso eng
dc.publisher Lippincott Williams & Wilkins
dc.relation.ispartof Hypertension
dc.rights Acesso aberto
dc.subject tubular renin en
dc.subject prorenin en
dc.subject Ang II-dependent hypertension en
dc.subject angiotensin II en
dc.subject renin mRNA en
dc.subject renin Western blot en
dc.subject renin immunostaining en
dc.title Collecting duct renin is upregulated in both kidneys of 2-kidney, 1-clip Goldblatt hypertensive rats en
dc.type Artigo
dc.contributor.institution Tulane Univ
dc.contributor.institution Ponce Sch Med
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Tulane Univ, Hlth Sci Ctr, Dept Physiol & Hypertens, New Orleans, LA 70112 USA
dc.description.affiliation Tulane Univ, Hlth Sci Ctr, Renal Ctr Excellence, New Orleans, LA 70112 USA
dc.description.affiliation Ponce Sch Med, Dept Physiol & Pharmacol, Ponce, PR USA
dc.description.affiliation Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Med, Div Nephrol, São Paulo, Brazil
dc.identifier.doi 10.1161/HYPERTENSIONAHA.108.110916
dc.description.source Web of Science
dc.identifier.wos WOS:000256053500033



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