Identification of new splice variants of the genes GAFF and BCMA
Smirnova, Anna S. [UNIFESP]
Andrade-Oliveira, Vinicius [UNIFESP]
Gerbase-DeLima, Maria [UNIFESP]
Is part ofMolecular Immunology
MetadataShow full item record
The TNF superfamily ligands BAFF and APRIL and receptors BCMA, TACT and BAFF-R play an important role in the regulation of B cell immunity. A number of functionally important splice isoforms have already been characterized for these molecules, stimulating the search for new transcript variants (TVs). Here we report two new BAFF TVs and three BCMA TVs, all potentially codifying new proteins. BAFF TVs were expressed in peripheral blood mononuclear cells (PBMC) of nearly all the individuals studied, decreasing in level when PBMC were activated by PMA and ionomycin. They were also detected in PBMC cytoplasmic RNA. Low levels of the BAFF TVs in all lymphocyte subpopulations analyzed suggest that their main source in PBMC are monocytes. BCMA TVs were observed only in some CD 19+ cell samples. Functional studies concerning interaction between isoforms of BAFF, APRIL and their receptors are needed for elucidation of their significance in the immune response. (c) 2007 Elsevier B.V. All rights reserved.
CitationMolecular Immunology. Oxford: Pergamon-Elsevier B.V., v. 45, n. 4, p. 1179-1183, 2008.
- EPE - Artigos