BmSI-7, a novel subtilisin inhibitor from Boophilus microplus, with activity toward Pr1 proteases from the fungus Metarhizium anisopliae

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dc.contributor.author Sasaki, Sergio D. [UNIFESP]
dc.contributor.author Lima, Cdssia A. de [UNIFESP]
dc.contributor.author Lovato, Diogo V. [UNIFESP]
dc.contributor.author Juliano, Maria A. [UNIFESP]
dc.contributor.author Torquato, Ricardo J. S. [UNIFESP]
dc.contributor.author Tanaka, Aparecida S. [UNIFESP]
dc.date.accessioned 2016-01-24T13:49:32Z
dc.date.available 2016-01-24T13:49:32Z
dc.date.issued 2008-02-01
dc.identifier http://dx.doi.org/10.1016/j.exppara.2007.08.003
dc.identifier.citation Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 118, n. 2, p. 214-220, 2008.
dc.identifier.issn 0014-4894
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/30414
dc.description.abstract BmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs. the inhibitors isolated by classical purification methods presented molecular masses of 7408 and 7271 Da, respectively, by MALDI-TOF-MS. Both BmSI-7 and BmSI-6 inhibited neutrophil elastase (K-i 0.4 and 0.3 nM) and subtilisin A (K-i 1.4 nM for both inhibitors). They also strongly inhibited Pr1 proteases from the fungus Metarhizium anisopliae; BmSI-7 (K-i 50 nM) and BmSI-6 (K-i 2.2 nM). the BmSI-7 full length cDNA was obtained using amino acid sequence information of BmSI-7 peptides generated by proteolytic digestion. BmSI-7 belongs to trypsin inhibitor like cysteine rich domain family (TIL), and it is transcribed in ovary, fat body, gut, salivary gland and haemocytes. BmSI-7 is the first TIL inhibitor described with inhibitory activity toward subtilisin A and Pr1 proteases of entomopathogenic fungi. (C) 2007 Elsevier Inc. All rights reserved. en
dc.format.extent 214-220
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Experimental Parasitology
dc.rights Acesso restrito
dc.subject Boophilus microplus en
dc.subject protease inhibitor en
dc.subject serine protease en
dc.subject egg proteins en
dc.subject tick en
dc.subject Metarhizium anisopliae protease en
dc.subject fungi en
dc.title BmSI-7, a novel subtilisin inhibitor from Boophilus microplus, with activity toward Pr1 proteases from the fungus Metarhizium anisopliae en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliation UNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifesp UNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifesp UNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, SP, Brazil
dc.identifier.doi 10.1016/j.exppara.2007.08.003
dc.description.source Web of Science
dc.identifier.wos WOS:000252982400010



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