Comparative evaluation of therapeutic DNA vaccines against Trypanosoma cruzi in mice

Comparative evaluation of therapeutic DNA vaccines against Trypanosoma cruzi in mice

Author Sanchez-Burgos, Gilma Google Scholar
Mezquita-Vega, R. Gabino Google Scholar
Escobedo-Ortegon, Javier Google Scholar
Ramirez-Sierra, Maria Jesus Google Scholar
Arjona-Torres, Arletty Google Scholar
Ouaissi, Ali Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Dumonteil, Eric Google Scholar
Institution Univ Autonoma Yucatan
INSERM
Universidade Federal de São Paulo (UNIFESP)
Abstract Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, is a major public health problem in most of Latin America. A key priority is the development of new treatments, due to the poor efficacy of current ones. We report here the comparative evaluation of therapeutic DNA vaccines encoding various T. cruzi antigens. ICR mice infected with 500 parasites intraperitoneally were treated at 5 and 12 days postinfection with 20 mu g of plasmid DNA encoding T. cruzi antigens TSA-1, TS, ASP-2-like, Tc52 or Tc24. Treatment with plasmid encoding TS and/or ASP-2-like antigens had no significant effect on parasitemia or survival. Treatment with Tc52 DNA significantly reduced parasitemia, as well as cardiac parasite burden, and improved survival, although myocarditis was not significantly affected. Finally, treatment with plasmids encoding Tc24 and TSA-1 induced the most complete control of disease as evidenced by significant reductions in parasitemia, mortality, myocarditis and heart parasite burden. These data demonstrate that therapeutic vaccine efficacy is dependent on the antigen and suggest that DNA vaccines encoding Tc24, TSA-1, and Tc52 represent the best candidates for further studies of a therapeutic vaccine against Chagas disease.
Keywords protozoa
chagas disease
therapeutic vaccine
immunotherapy
Language English
Date 2007-08-01
Published in Fems Immunology and Medical Microbiology. Malden: Wiley-Blackwell, v. 50, n. 3, p. 333-341, 2007.
ISSN 0928-8244 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 333-341
Origin http://dx.doi.org/10.1111/j.1574-695X.2007.00251.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000247817800008
URI http://repositorio.unifesp.br/handle/11600/29886

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