DNA damage and nitric oxide synthesis in experimentally infected balb/c mice with Trypanosoma cruzi

DNA damage and nitric oxide synthesis in experimentally infected balb/c mice with Trypanosoma cruzi

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Calvi, Sueli A. Google Scholar
Picka, Mariele M. Google Scholar
Persi, Eliana Google Scholar
Carvalho, Thais B. de Google Scholar
Caetano, Priscila K. Google Scholar
Nagoshi, Lidiana R. Google Scholar
Lima, Carlos R. G. Google Scholar
Machado, Jussara Marcondes Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Nacl Estadual São Paulo
Abstract This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals); Trypanosoma cruzi infected; T cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. the results obtained with the single cell gel (comet) assay showed that T cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly, T cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (p < 0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the T cruzi infected group. Taken together, these results indicate that T cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced T. cruzi genotoxic effects in spleen cells. (c) 2006 Elsevier Inc. All rights reserved.
Keywords Trypanosoma cruzi
Chagas disease
Single cell gel (comet) assay
Nitric oxide
Language English
Date 2007-07-01
Published in Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 116, n. 3, p. 296-301, 2007.
ISSN 0014-4894 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 296-301
Origin https://dx.doi.org/10.1016/j.exppara.2006.12.007
Access rights Closed access
Type Article
Web of Science ID WOS:000247662500014
URI https://repositorio.unifesp.br/handle/11600/29856

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