Biocompatibility of gutta-percha solvents using in vitro mammalian test-system

Biocompatibility of gutta-percha solvents using in vitro mammalian test-system

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Matsumoto, Mariza Akemi Google Scholar
Marques, Mariangela Esther Alencar Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
São Paulo State Univ
Abstract Objectives. Taking into consideration that DNA damage and cellular death play important roles during carcinogenesis, the purpose of the present study was to evaluate in vitro genotoxic or cytotoxic effects of chloroform and eucalyptol by single cell gel (comet) assay and trypan blue exclusion test, respectively.Study design. Chloroform and eucalyptol were exposed to Chinese hamster ovary cells in culture directly for 3 hours at 37 degrees C at final concentrations ranging from 1.25 to 10 mu L/mL. the negative control group was treated with vehicle control (phosphate-buffered solution), and the positive control group was treated with methyl metasulfonate (MMS, at 1 mu g/mL concentration). All data were analyzed by the Kruskal-Wallis nonparametric test followed by the Dunn test.Results. the results showed that both gutta-percha solvents were cytotoxic at concentrations of 2.5, 5, and 10 mu L/mL (P < .05). On the other hand, both solvents did not induce DNA breakage at 1.25 mu L/mL concentration.Conclusions. These results suggest that both chloroform or eucalyptol are strong cytotoxicants, but they may not be a factor that increases the level of DNA lesions in mammalian cells.
Language English
Date 2007-05-01
Published in Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. New York: Mosby-Elsevier, v. 103, n. 5, p. E106-E109, 2007.
ISSN 1079-2104 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent E106-E109
Access rights Closed access
Type Article
Web of Science ID WOS:000248082300019

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