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Purification and partial characterization of human neutrophil elastase inhibitors from the marine snail Cenchritis muricatus (Mollusca)

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Date
2007-04-01
Author
Gonzalez, Yamile
Tanaka, Aparecida S.
Hirata, Izaura Y.
Alonso del Rivero, Maday
Oliva, Maria L. V.
Araujo, Mariana S.
Chavez, Maria A.
Type
Artigo
ISSN
1095-6433
Is part of
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology
DOI
10.1016/j.cbpa.2006.01.022
Metadata
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Abstract
Human neutrophil elastase inhibition was detected in a crude extract of the marine snail Cenchritis muricatus (Gastropoda, Mollusca). This inhibitory activity remained after heating this extract at 60 C for 30min. From this extract, three human neutrophil elastase inhibitors (designated CmPI-I, CmPI-II and CmPI-III) were purified by affinity and reversed-phase chromatographies. Homogeneity of CmPI-I and CmPI-II was confirmed, while CmPI-III showed a single peak in reversed-phase chromatography, but heterogeneity in SDS-PAGE with preliminary molecular masses in the range of 18.4 to 22.0kDa. in contrast, MALDI-TOF mass spectrometry of CmPI-I and CmPI-II showed that these inhibitors are molecules of low molecular mass, 5576 and 5469 Da, respectively. N-terminal amino acid sequences of CmPI-I (6 amino acids) and CmPI-II (20 amino acids) were determined. Homology to Kazal-type protease inhibitors was preliminarily detected for CmPI-II. Both inhibitors, CmPI-I and CmPI-II are able to inhibit human neutrophil elastase strongly, with equilibrium dissociation constant (K;) values of 54.2 and 1.6nM, respectively. in addition, trypsin and pancreatic elastase were also inhibited, but not plasma kallikrein or thrombin. CmPI-I and CmPI-II are the first human neutrophil elastase inhibitors described in a mollusk. (c) 2006 Elsevier Inc. All rights reserved.
Citation
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology. New York: Elsevier B.V., v. 146, n. 4, p. 506-513, 2007.
Keywords
Cenchritis muricatus
human neutrophil elastase
marine invertebrate
Mollusca
protease inhibitors
URI
http://repositorio.unifesp.br/handle/11600/29620
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  • EPM - Artigos [17709]

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