Carcinoembryonic antigen (CEA) mimicry by an anti-idiotypic scFv isolated from anti-Id 6.C4 hybridoma
Pignatari, Graciela Conceicao
Parise, Carolina Bellini
Soares, Fernando Augusto
Moraes, Jane Zveiter de
Han, Sang Won
Is part ofJournal of Biotechnology
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Since carcinoembryonic antigen (CEA) is expressed during embryonic life, it is not immunogenic in humans. the use of anti-idiotypic (Id) antibodies as a surrogate of antigen in the immunization has been considered a promising strategy for breaking tolerance to some tumor associated antigens. We have described an anti-Id monoclonal antibody (MAb), designated 6.C4, which is able to mimic CEA functionally. the anti-Id MAb 6.C4 was shown to elicit antibodies that recognized CEA in vitro and in vivo. in the present study, we sought to verify whether a single chain (scFv) antibody obtained, the scFv 6.C4, would retain the ability to mimic CEA. Two scFv containing the variable heavy and light chain domains of 6.C4 were constructed with a 15-amino acid linker: one with and another without signal peptide. DNA immunization of mice with both forms of scFv individually elicited antibodies able to recognize CEA. (c) 2006 Elsevier B.V. All rights reserved.
CitationJournal of Biotechnology. Amsterdam: Elsevier B.V., v. 127, n. 4, p. 615-625, 2007.
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