Biocompatibility of glass-ionomer cements using mouse lymphoma cells in vitro

Biocompatibility of glass-ionomer cements using mouse lymphoma cells in vitro

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Marques, Mariangela Esther Alencar Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Glass-ionomer cements are widely used in dentistry as restorative materials and adhesives for composite restorations. A number of genotoxicity studies have been conducted using these materials with results conflicting so far. Thus, the approach was aimed to look at the genotoxic and cytotoxic potential of three different glass-ionomer cements available commercially (Ketac Cem, Ketac Molar and Vitrebond) by the single cell gel (comet) assay and trypan blue exclusion test, respectively. for this, such materials were exposed to mouse lymphoma cells in vitro for 1 h at 37 degrees C. Data were assessed by Kruskall-Wallis non-parametric test. the results showed that all powders assayed did not show genotoxic effects. On the other hand, the liquid from Vitrebond at 0.1% dilution caused an increase of DNA injury. Significant statistically differences (P < 0.05) in cytotoxicity provoked by all powders tested were observed for exposure at 1000 mu g mL(-1) concentration and 100 mu g mL(-1) for Ketac Molar. With respect to liquids of glass-ionomer cements evaluated, the major toxic effect on cell viability was produced at 1%, beginning at the dilution of 0.5% for Vitrebond. Taken together, these results support the notion that some components of glass-ionomer cements show both genotoxic and cytotoxic effects in higher concentrations.
Keywords Cytotoxicity
Glass-ionomer cements
Mouse lymphoma cells
Language English
Date 2006-12-01
Published in Journal of Oral Rehabilitation. Oxford: Blackwell Publishing, v. 33, n. 12, p. 912-917, 2006.
ISSN 0305-182X (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 912-917
Access rights Closed access
Type Article
Web of Science ID WOS:000242235400006

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