Modulation of kinin B1 receptor expression by endogenous angiotensin II in hypertensive rats

Modulation of kinin B1 receptor expression by endogenous angiotensin II in hypertensive rats

Author Fernandes, Liliarn Google Scholar
Ceravolo, Graziela S. Google Scholar
Fortes, Zuleica B. Google Scholar
Tostes, Rita Google Scholar
Santos, Robson A. S. Google Scholar
Santos, Jerusa A. Google Scholar
Mori, Marcelo A. S. Google Scholar
Pesquero, Joao Bosco Google Scholar
Carvalho, Maria Helena C. de Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Abstract We investigated the expression and localization of B I receptor in tissues of rats submitted to a renin-dependent model of hypertension (2K-IC), and analyzed the influence of endogenous Ang II in modulating the in vivo expression of these receptors. B1 mRNA levels in the heart, kidney and thoracic aorta were quantified by real time PCR, B1 receptor protein expression was assessed by immunchistochemistry, plasma Ang II levels were analyzed by radioirnmunoassay and the effects of ATI receptor blockade were determined after losartan treatment. 2K-IC rats presented a marked increase in Ang II levels when compared to sham-operated rats. in parallel, cardiac- (but not renal and aortic) B I mRNA levels were 15-fold higher in 2K-IC than in sham rats. in 2K-IC, B1 expression was detected in the endothelium of small cardiac arteries and in cardiomyocytes. Losartan completely reverted the increased B1 mRNA levels and significantly decreased the protein expression observed in 2K-IC rats, despite reducing, but not normalizing blood pressure. We conclude that in the 2K-IC rat, induction of cardiac B I receptor might be tightly linked to ATI receptor activation. These data suggest the existence of a new site of interaction between kinins and angiotensins, and might provide important contributions for a better understanding of the pathophysiology of hypertension. (c) 2006 Elsevier B.V. All rights reserved.
Keywords B1 receptor
AT1 receptor
2 kidneys-1 clip
hypertension
losartan
Language English
Date 2006-09-11
Published in Regulatory Peptides. Amsterdam: Elsevier B.V., v. 136, n. 1-3, p. 92-97, 2006.
ISSN 0167-0115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 92-97
Origin http://dx.doi.org/10.1016/j.regpep.2006.04.018
Access rights Closed access
Type Article
Web of Science ID WOS:000241177200013
URI http://repositorio.unifesp.br/handle/11600/29155

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