Ischemic preconditioning prevents apoptotic cell death and necrosis in early and intermediate phases of liver ischemia-reperfusion injury in rats

Ischemic preconditioning prevents apoptotic cell death and necrosis in early and intermediate phases of liver ischemia-reperfusion injury in rats

Author Quireze Junior, Claudemiro Autor UNIFESP Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Leitao, Regina Maria Cubero Autor UNIFESP Google Scholar
Juliano, Yara Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Poli de Figueiredo, Luiz Francisco Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Ischemic preconditioning (IPC) may be useful in attenuating the hepatic ischemia reperfusion (IR) syndrome by means of improving cell resistance to anoxia and reoxygenation and preventing cell death. Since there are insufficient data available regarding the chronology of preconditioning effects, we investigated the role of IPC, to test the hypothesis that liver protection would occur during the early and intermediate phases of the reperfusion period. Wistar rats ( n = 72) were randomly assigned into six experimental groups, 12 animals each. A 40-min ischemia to the left lateral and median liver lobes was induced by selective hepatic pedicle clamping followed by 30 min or 240 min of reperfusion (IR30 and IR240). IPC groups (IPC30 and IPC240) underwent a 10 min of ischemia followed by 10 min of reperfusion preceding the definitive 40-min ischemic period. Sham-operated animals were followed for 30 and 240 min. Hepatic enzymes and histological evaluation were performed after the reperfusion period. Hepatic ischemia-reperfusion (IR30 and IR240) induced marked increases in liver enzymes levels after 30 min and particularly after 240 min. IPC effectively attenuated those enzymatic increases. Microvesicular steatosis was observed after 30 min, but not 240 min, of reperfusion in both IPC and IR livers. Necrosis was detected in 66.7% of IR240 and only in 8.3% of IPC240. Both hepatocyte and sinusoidal apoptosis were markedly attenuated by IPC. We conclude that IPC provided protection against hepatic ischemia reperfusion injury in early and intermediate phases of the reperfusion period, reducing hepatic enzymatic leakage and ameliorating hepatic apoptosis and necrosis.
Keywords animal experimentation
apoptosis
ischemia
ischemic preconditioning
liver
liver transplantation
reperfusion injury
Language English
Date 2006-07-01
Published in Journal of Investigative Surgery. Philadelphia: Taylor & Francis Inc, v. 19, n. 4, p. 229-236, 2006.
ISSN 0894-1939 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 229-236
Origin http://dx.doi.org/10.1080/08941930600778206
Access rights Closed access
Type Article
Web of Science ID WOS:000238990600004
URI http://repositorio.unifesp.br/handle/11600/29032

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