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dc.contributor.authorParedes-Gamero, E. J.
dc.contributor.authorCraveiro, R. B.
dc.contributor.authorPesquero, J. B.
dc.contributor.authorFranca, J. P.
dc.contributor.authorOshiro, MEM
dc.contributor.authorFerreira, A. T.
dc.date.accessioned2016-01-24T12:41:02Z
dc.date.available2016-01-24T12:41:02Z
dc.date.issued2006-03-18
dc.identifierhttp://dx.doi.org/10.1016/j.ejphar.2006.01.010
dc.identifier.citationEuropean Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 534, n. 1-3, p. 30-38, 2006.
dc.identifier.issn0014-2999
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28793
dc.description.abstractIn this study, we describe the presence of P2 receptor subtypes and Ca2+ signaling in erythroblasts. ATP and ADP produced a biphasic increase of intracellular Ca2+ concentration ([Ca2+],), with an initial transient phase followed by a sustained phase. Reverse transcription polymerase chain reaction (RT-PCR) showed the expression of P2Y(1), P2Y(2) and P2Y(12). the selective P2Y(1) receptor antagonist 2'-deoxy-N-6-methyl-adenosine- 3',5'diphosphate (MRS2179) and the Gi protein inhibitor pertussis toxin blocked Ca2+ increase. the initial transient [Ca2+](i) increase phase was sensitive to the 1,4,5-inositol trisphosphate (IP3) receptor blocker 2-aminoethoxy-diphenylborate (2-APB), while the sustained phase was sensitive to the protein kinase C (PKC) inhibitor 2-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3(1H-indol-3-yl)-maleimide (GF109203X) and calcium calmodulin kinase 11 (CaMKII) inhibitor 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62). in addition, the PKC activator phorbol-12,13-dibutyrate (PDBu) produced increase of [Ca2+](i). Flow cytometry analysis showed the expression of Ca2+-dependent PKC alpha, beta I, gamma and phospho-CaMKII.These results suggest that the activation of the P2Y(1) receptor triggers two different [Ca2+]i increase pathways, one IP3-dependent and the other kinase-dependent. (c) 2006 Elsevier B.V. All rights reserved.en
dc.format.extent30-38
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEuropean Journal of Pharmacology
dc.rightsAcesso restrito
dc.subjectbone marrowen
dc.subjecterythroblasten
dc.subjectP2Y(1)en
dc.subjectreceptoren
dc.subjectintracellular calciumen
dc.subjectCaMKIIen
dc.subjectPKCen
dc.titleActivation of P2Y(1) receptor triggers two calcium signaling pathways in bone marrow erythroblastsen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.identifier.doi10.1016/j.ejphar.2006.01.010
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000236409600004


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