An unexpected inhibitory activity of Kunitz-type serine proteinase inhibitor derived from Boophilus microplus trypsin inhibitor on cathepsin L

An unexpected inhibitory activity of Kunitz-type serine proteinase inhibitor derived from Boophilus microplus trypsin inhibitor on cathepsin L

Author Sasaki, S. D. Google Scholar
Cotrin, S. S. Google Scholar
Carmona, A. K. Google Scholar
Tanaka, A. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Several BPTI-Kunitz-type serine proteinase inhibitors were described in tick Boophilus microplus and Rhipicephalus sanguineus species. in this work, we present a synthetic gene based on two tick BPTI-Kunitz-type serine proteinase inhibitors, the first domain of B. micro-plus trypsin inhibitor-A (BmTI-A) and the carrapatin, the inhibitors were named BmTIsint and BmTIsint Mut. Our present results showed that BmTIsint and BmTIsint Mut inhibited trypsin (K-i 3.3 and 1.0 nM) and human plasma kallikrein (K-i 16.5 and 35 nM), but in contrast to BmTI-A, the inhibitors did not inhibit human neutrophil elastase. BmTIsint was able to produce immunological response in mice but not in bovines. in addition, it is the first description of a BPTI-Kunitz-type inhibitor as a cysteine proteinase inhibitor, BmTIsint apparent dissociation constant (Ki) for cathepsin L was 108 nM. Our findings open the possibility up to obtain new molecules as potent serine or cysteine proteinase inhibitors using BmTIsint as a model. (c) 2006 Elsevier Inc. All rights reserved.
Keywords cysteine proteinase inhibitor
serine proteinase inhibitor
tick
ectoparasite
Boophilus microplus
Language English
Date 2006-03-03
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 341, n. 1, p. 266-272, 2006.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 266-272
Origin http://dx.doi.org/10.1016/j.bbrc.2005.12.178
Access rights Closed access
Type Article
Web of Science ID WOS:000235313400039
URI http://repositorio.unifesp.br/handle/11600/28790

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