Melanocyte transformation associated with substrate adhesion impediment

Show simple item record

dc.contributor.author Oba-Shinjo, Sueli Mieko [UNIFESP]
dc.contributor.author Correa, Mariangela [UNIFESP]
dc.contributor.author Ricca, Tatiana Iervolino [UNIFESP]
dc.contributor.author Molognoni, Fernanda [UNIFESP]
dc.contributor.author Pinhal, Maria Aparecida da Silva [UNIFESP]
dc.contributor.author Neves, Izabel A. [UNIFESP]
dc.contributor.author Marie, Sueli Kazue Nagahashi
dc.contributor.author Sampaio, Lucia de Oliveira [UNIFESP]
dc.contributor.author Nader, Helena Bonciani [UNIFESP]
dc.contributor.author Chammas, Roger
dc.contributor.author Jasiulionis, Miriam Galvonas [UNIFESP]
dc.date.accessioned 2016-01-24T12:41:00Z
dc.date.available 2016-01-24T12:41:00Z
dc.date.issued 2006-03-01
dc.identifier http://dx.doi.org/10.1593/neo.05781
dc.identifier.citation Neoplasia. Ann Arbor: Neoplasia Press, v. 8, n. 3, p. 231-241, 2006.
dc.identifier.issn 1522-8002
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28761
dc.description.abstract Exclude experimental models of malignant transformationemploy chemical and physical carcinogens or genetic manipulations to study tumor progression. in this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of forced anchorage impediment. the great majority of these cells underwent anoikis when maintained in suspension. After one deadhesion cycle, phenotypic alterations were noticeable in the few surviving cells, which became more numerous and showed progressive alterations after each adhesion impediment step. No significant differences in cell surface expression of integrins were detected, but a clear electrophoretic migration shift, compatible with an altered glycosylation pattern, was observed for beta(1) chain in transformed cell lines. in parallel, a progressive enrichment of tri- and tetra-antennary N-glycans was apparent, suggesting increased N-acetylglucosaminyl-transferase V activity. Alterations both in proteoglycan glycosylation pattern and core protein expression were detected during the transformation process. in conclusion, this model corroborates the role of adhesion state as a promoting agent in transformation process and demonstrates that cell adhesion disturbances may act as carcinogenic stimuli, at least for a nontumorigenic immortalized melanocyte lineage. These findings have intriguing implications for in vivo carcinogenesis, suggesting that anchorage independence may precede, and contribute to, neoplastic conversion. en
dc.format.extent 231-241
dc.language.iso eng
dc.publisher Neoplasia Press
dc.relation.ispartof Neoplasia
dc.rights Acesso aberto
dc.subject melanocyte transformation en
dc.subject substrate adhesion impediment en
dc.subject adhesion molecules en
dc.subject N-glycans en
dc.subject proteoglycans en
dc.title Melanocyte transformation associated with substrate adhesion impediment en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Micro Imuno & Parastiol, Disciplina Imunol, BR-04023900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Dept Neurol, Mol Biol Lab, BR-05508 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Med, Expt Oncol Lab, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Micro Imuno & Parastiol, Disciplina Imunol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1593/neo.05781
dc.description.source Web of Science
dc.identifier.wos WOS:000239282800009



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account