Testosterone-immunopositive primordial germ cells in the testis of the bullfrog, Rana catesbeiana

Testosterone-immunopositive primordial germ cells in the testis of the bullfrog, Rana catesbeiana

Author Sasso-Cerri, Estela Autor UNIFESP Google Scholar
Freymüller-Haapalainen, Edna Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Institution Univ Estadual São Paulo UNESP
Universidade Federal de São Paulo (UNIFESP)
Abstract In amphibia, steroidogenesis remains quiescent in distinct seasonal periods, but the mechanism by which spermatogenesis is maintained under low steroidogenic conditions is not clear. in the present study, testosterone location in the testes of Rana catesbeiana was investigated immunohistochemically during breeding (summer) and nonbreeding (winter) periods. in winter, the scarce interstitial tissue exhibited occasional testosterone immunopositivity in the interstitial cells but the cytoplasm of primordial germ cells (PG cells) was clearly immunopositive. By contrast, in summer, PG cells contained little or no immunoreactivity whereas strong immunolabelling was present in the well-developed interstitial tissue. These results suggest that PG cells could retain testosterone during winter. This androgen reservoir could be involved in the control of early spermatogenesis in winter and/or to guarantee spermiogenesis and spermiation in the next spring/summer. the weak or negative immunoreaction in the summer PG cells might reflect consumption of androgen reservoir by the intense spermatogenic activity from spring to summer. Thus, besides acting as stem cells, PG cells of R. catesbeiana could exert an androgen regulatory role during seasonal spermatogenesis.
Keywords amphibia
androgen
immunohistochemistry
interstitial cells
seasonal spermatogenesis
stem cells
Language English
Date 2005-06-01
Published in Journal of Anatomy. Oxford: Blackwell Publishing, v. 206, n. 6, p. 519-523, 2005.
ISSN 0021-8782 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 519-523
Origin http://dx.doi.org/10.1111/j.1469-7580.2005.00419.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000229581000001
URI http://repositorio.unifesp.br/handle/11600/28315

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