Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections

Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections

Author Lesenechal, Mylene Google Scholar
Becquart, Laurence Google Scholar
Lacoux, Xavier Google Scholar
Ladaviere, Laurent Google Scholar
Baida, Renata Cristina Pardos Autor UNIFESP Google Scholar
Paranhos-Baccala, Glaucia Google Scholar
Silveira, Jose Franco da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
CNRS bioMerieux
Univ Lyon 1
Abstract Tc40 is an immunodominant antigen present in natural Trypanosoma cruzi infections. This immunogen was thoroughly mapped by using overlapping amino acid sequences identified by gene cloning and chemical peptide synthesis. To map continuous epitopes of the Tc40 antigen, an epitope expression library was constructed and screened with sera from human chagasic patients. A major, linear B-cell epitope spanning residues 403 to 426 (PAKAAAPPAA) was identified in the central domain of Tc40. A synthetic peptide spanning this region reacted strongly with 89.8% of the serum samples from T. cruzi-infected individuals. This indicates that the main antigenic site is defined by the linear sequence of the peptide rather than a conformation-dependent structure. the major B-cell epitope of Tc40 shares a high degree of sequence identity with T. cruzi ribosomal and RNA binding proteins, suggesting the existence of cross-reactivity among these molecules.
Language English
Date 2005-02-01
Published in Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 12, n. 2, p. 329-333, 2005.
ISSN 1071-412X (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 329-333
Origin http://dx.doi.org/10.1128/CDLI.12.2.329-333.2005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000231196400014
URI http://repositorio.unifesp.br/handle/11600/28127

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